An investigation on purification and characterization of amyloglucosidase enzyme from Endomycopsis fibuligera by fermentation of sago starch has been carried out. This enzyme is inductive and can be produced by fermenting sago starch in a medium containing E. fibuligera. Crude enzyme was obtained by centrifuging the medium cultures containing E. fibuligera at 6,000 rpm for 20 min and then addin...

Actinomycetes are an uncommon agent of human infections and its pathogenic factors are not known. The present study reports a rare case isolation of an actinomycete from a woman pleural fluid; the strain was identified by 16S rRNA gene sequence analysis. This strain was tested to produce an extracellular protease that hydrolysis gelatin, casein and hemoglobin on agar mediums. The purification o...

Cellulase production by a Cellulomonas sp., isolated in 1985 from forest humus soil along the border of the Caspian Sea in Iran, was investigated. This strain secreted endo-and exo-cellulases in the culture medium, but some of the enzymes produced remained cell membrane bound. Cell bound enzymes were released by various treatments. The highest amount of endo-glucanase (up to 95%) and exo-gl...

Human erythrocyte transketolase (sedoheptulose-7-phosphate: D-glyceraldehyde-3-phosphate, glycolaldehyde transferase, E.C. 2.2.1.1.) has been isolated from erythrocytes with a specific activity of 59.84 U/mg. SDS-PAGE and SE-HPLC were used both as a measure of purity and as a preparative mean to obtain a higher degree of purity. Four protein bands corresponding to molecular weights of 32,0...

In this paper, we explore the effects of certain forbidden substructure conditions on preordered sets. In particular, we characterize in terms of these conditions those preordered sets which can be represented as the supremum of a well-ordered ascending chain of lowersets whose members are constructed by means of alternating applications of disjoint union and ordinal sums with chains. These dec...

Biosurfactants can be classified by their chemical composition and their origin. This review briefly describes various classes of biosurfactants based on their origin and introduces a few of the most widely used biosurfactants. The current status and future trends in biosurfactant production are discussed, with an emphasis on those derived from plants. Following a brief introduction of the prop...

The graph theoretic problem of identifying the finite sets C of constraint graphs for which there is a countable universal C-free graph is closely related to the problem of determining for which sets C the model companion T ∗ C of the theory of C-free graphs is א0-categorical, and this leads back to combinatorics. Little is known about these theories from any other perspective, such as stabilit...

The characterization of strongly perfect graphs by a restricted list of forbidden induced subgraphs has remained an open question for a long time. The minimal strongly imperfect graphs which are simultaneous imperfect are only odd holes and odd antiholes ( E. Olaru, [9]), but the entire list is not known, in spite of a lot of particular results in this direction. In this paper we give some new ...

Let L3 be the class of edge intersection graphs of linear 3-uniform hypergraphs. The problem of recognizing G ∈ L3 is NP-complete. Denote by δALG the minimal integer such that the problem ”G ∈ L3 ” is polynomially solvable in the class of graphs G with the minimal vertex degree δ(G) ≥ δALG and by δFIS the minimal integer such that L3 can be characterized by a finite list of forbidden induced su...

Antibody use is ubiquitous in the biomedical sciences. However, determining best research practices has not been trivial. Many commercially available antibodies and antibody-conjugates are poorly characterized and lack proper validation. Uncritical application of such useless tools has contributed to the reproducibility crisis in biomedical research. Despite early initiatives such as MIAPAR or ...