The enzymatic co-polymerization of modified nucleoside triphosphates (dN*TPs and N*TPs) is a versatile method for the expansion and exploration of expanded chemical space in SELEX and related combinatorial methods of in vitro selection. This strategy can be exploited to generate aptamers with improved or hitherto unknown properties. In this review, we discuss the nature of the functionalities a...

Family X DNA polymerases (PolXs) are involved in DNA repair. Their binding to gapped DNAs relies on two conserved helix-hairpin-helix motifs, one located at the 8-kDa domain and the other at the fingers subdomain. Bacterial/archaeal PolXs have a specifically conserved third helix-hairpin-helix motif (GFGxK) at the fingers subdomain whose putative role in DNA binding had not been established. He...

Dopamine (DA) has attracted extensive interest due to not only its important roles in physiological and pathological processes, but also its prospective applications in chemistry and materials science. In this work, we demonstrate that the urease catalytic reaction is an effective new approach for a better control of DA polymerization to polydopamine nanoparticles (PDA NPs). And we further deve...

This study explores the use of α-1,3-glucan, which can be produced through an enzymatic polymerization process, as additive for thermoplastic polyurethane composite systems. Most importantly, highly filled composites achieved with up to 80% by weight polysaccharide content. Analysis mechanical properties demonstrates that strength and modulus significantly improved, while plasticizer content al...

Currently, most commodity chemicals and polymers are manufactured from nonrenewable petroleum-based resources. However, due to its finite nature social claims about environment preservation, alternative sources of value-added building block being intensively studied. Renewable lignocellulosic biomass has offered an attractive replacement for fossil-based chemicals. Lignin C5/C6 sugars obtained ...

Porang (Amorphophallus oncophyllus) is an indigenous tuber of Indonesia that rich in glucomannan. An alternative approach to produce porang oligo-glucomannan (POG) as prebiotic from glucomannan (PGM) was made by enzymatic hydrolysis using β-mannanase. This study aimed POG under optimal conditions controlled process. The PGM flour contained 96.12% indigestible carbohydrates. optimum condition pr...

Resistant starch type 3 (RS-3) holds great potential as a prebiotic by supporting gut microbiota following intestinal digestion. However the factors influencing digestibility of RS-3 are largely unknown. This research aims to reveal how crystal and molecular weight (distribution) influence its resistance. Narrow polydisperse ?-glucans degree polymerization (DP) 14–76, either obtained enzymatic ...

atom transfer radical polymerization (atrp) of styrene was carried out at 105°c and a monte carlo simulation was employed to model the system. the variations of monomer conversion, the initiator concentration, average molecular weight, and molecular weight distribution were evaluated as the reaction proceeded. according to the results obtained, for similar reaction time, monomer conversion is h...