The principal objective of this study was to determine the in vitro antibacterial activity of the water-soluble protein enzymatic hydrolysates and the ethanol (EtOH)-extracted fraction obtained from fly-maggots (Musca domestica L.) against MRSA (methicillinresistant Staphylococcus aureus) strains and VRE (Vancomycin-resistant enterococci) 5117 strain. The water soluble protein enzymatic hydroly...

A flow-through biosensor consisting of a fixed bed bioreactor was employed to detect the insecticideparaoxon. Based on the inhibition of organophosphorous insecticide to the enzymatic activity of acetylcholinesterase (AChE), using paraoxon as a model compound, the condition for detection of the insecticide were optimized. The influence of enzyme loading on the packing surface was studied ...

Tyrosinase is a key enzyme for melanin biosynthesis, catalyzing the oxidation of L-tyrosine to L-dopaquinone. The tyrosinase inhibition is an effective approach to control hyperpigmentation in human skin and enzymatic browning in fruits and vegetables. Kojic acid is a naturally-occurring tyrosinase inhibitor which has been clinically used to treat the hyperpigmentation of skin. However, kojic a...

The hepatitis C virus (HCV) nonstructural 3 protein (NS3) contains at least two domains associated with multiple enzymatic activities; a serine protease activity resides in the N-terminal one-third of the protein, whereas RNA helicase activity and RNA-stimulated nucleoside triphosphatase activity are associated with the C-terminal portion. To study the possible mutual influence of these enzymat...

The present study was aimed to enzymatic antioxidants (superoxide dismutase, catalase, polyphenol oxidase), nonenzymatic (saponin, flavonoids, ascorbic acid and tannin), anti-oxidant ability assays (total anti-oxidant, hydroxyl radical scavenging ability, reducing power activity, inhibition of lipid peroxide formation, DPPH activity metal chelating assay) in I. marginata leaves whole plant. res...

Interest in the synthesis and application of thymol esters has increased recent years due to numerous applications associated with its biological activities. The enzymatic octanoate by esterification octanoic acid was explored using soluble lipases immobilized lipase biocatalysts solvent-free systems. Candida antarctica B form most active biocatalyst for this reaction. Different feeding strateg...

Nitric oxide generation in brain cytosolic fractions markedly enhances ADP-ribosylation of a single 37-kDa protein. By utilizing a biotinylated NAD and avidin affinity chromatography, we purified this protein. Partial amino acid sequencing establishes its identity as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). This is further confirmed by detection of GAPDH enzymatic activity in the purif...

Drummond, Margaret C. (Emory University, Atlanta, Ga.) and Morris Tager. Enzymatic activities associated with clotting of fibrinogen by staphylocoagulase and coagulase-reacting factor and their inhibition by diisopropylfluorophosphate. J. Bacteriol. 83:975-980. 1962.-The chemical mechanism of fibrinogen clotting by staphylocoagulase and its plasma factor (CRF) involves a preliminary stage of pr...

Because conventional approaches for evaluating sugar release from the coupled operations of pretreatment and enzymatic hydrolysis are extremely time and material intensive, high throughput (HT) pretreatment and enzymatic hydrolysis systems have become vital for screening large numbers of lignocellulosic biomass samples to identify feedstocks and/or processing conditions that significantly impro...

In the current work, a model for enzymatic hydrolysis and SSF has been reviewed and modified. Parameters referring to the enzymatic hydrolysis were estimated against new experimental data by using a non-linear least square regression tool in Matlab (MathWorks Inc.). To enable modeling of different substrates a new parameter accounting for different substrates susceptibility to enzymatic hydroly...