Most surgeons seem to favour the former method. Even when the erisophake is used instead of forceps, the technique of zonulolotomy and delivery of the lens remains essentially the same. Since the introduction of A.C.T., however, mechanical zonulolotomy has been replaced by enzymatic zonulolysis and the extraction of the lens by seizing it at the 12 o'clock position is being increasingly practis...

We demonstrate a novel assay for physicochemical extraction and isotachophoresis-based purification of 16S rRNA from whole human blood infected with Pseudomonas putida . This on-chip assay is unique in that the extraction can be automated using isotachophoresis in a simple device with no moving parts, it protects RNA from degradation when isolating from ribonuclease-rich matrices (such as bloo...

INTRODUCTION Biological samples, pharmaceuticals or food contain proteins, lipids, polymers, ammoniums and macromolecules that alter the detection of infectious agents by DNA amplification techniques (PCR). Moreover the targeted DNA has to be released from the complex cell walls and the compact nucleoprotein matrixes and cleared from potential inhibitors. The goal of the present work was to ass...

Cultivation-independent investigation of microbial ecology is biased by the DNA extraction methods used. We aimed to quantify those biases by comparative analysis of the metagenome mined from four diverse naturally fermented foods (bamboo shoot, milk, fish, soybean) using eight different DNA extraction methods with different cell lysis principles. Our findings revealed that the enzymatic lysis ...

Rapid isolation of high-purity microbial genomic DNA is necessary for genome analysis. In this study, the authors compared a one-hour procedure using a microwave with enzymatic and boiling methods of genomic DNA extraction from Gram-negative and Gram-positive bacteria. High DNA concentration and purity were observed for both MRSA and ESBL strains (80.1 and 91.1 μg/ml; OD260/280, 1.82 and 1.70, ...

a long-term study was conducted between 1985 and 2003 on rehabilitation trials at sishen ironore mine, south africa, to identify grass species that would survive in the artificial growth medium appliedto the sites, and to determine the most suitable medium for sustainable vegetation growth. vegetationestablishment was tested at slopes of 18° and 34° and with five different cover materials. afte...

C-Phycocyanin is a natural blue dye used in food and pharmaceutical industry. In the present study, a simple and efficient method to extract C-phycocyanin from Spirulina platensis wet biomass is reported. The extractions were carried out using six different methods, including chemical (organic and inorganic acid treatment), physical (freezing and thawing, sonication, homogenization) and enzymat...

The interest of supplying lignocellulosic materials for producing fermentable sugars has recently emerged in order to diminish the negative environmental effects fossil fuels. In this study, Ulmus minor clone Ademuz, characterized its tolerance Dutch elm disease and rapid growth, was evaluated as a source sugars. For that, different pretreatments, comprising autohydrolysis, dilute acid hydrolys...