The main transmission pathway of Helicobacter pylori has not been determined, but several reports have described detection of H. pylori DNA in drinking and environmental water, suggesting that H. pylori may be waterborne. To address this possibility, we developed, tested, and optimized two complementary H. pylori-specific real-time PCR assays for quantification of H. pylori DNA in water. The mi...

Pepper mild mottle virus (PMMoV) is a plant virus that has been recently proposed as a potential indicator of human fecal contamination of environmental waters; however, information on its geographical occurrence in surface water is still limited. We aimed to determine the seasonal and geographic occurrence of PMMoV in drinking water sources all over Japan. Between July 2008 and February 2011, ...

This study aimed at evaluating potential differences among the bacterial communities from formation water and oil samples originated from biodegraded and non-biodegraded Brazilian petroleum reservoirs by using a PCR-DGGE based approach. Environmental DNA was isolated and used in PCR reactions with bacterial primers, followed by separation of 16S rDNA fragments in the DGGE. PCR products were als...

This work describes the development, optimization, and validation of a new method for the simultaneous determination of a wide range of pharmaceuticals (beta-blockers, lipid regulators…) and personal care products (fragrances, UV filters, phthalates…) in both aqueous and solid environmental matrices. Target compounds were extracted from sediments using pressurized hot water extraction followed ...

A rapid and simple method of using solid-phase microextraction was developed for determination of 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in water samples. In this method, the target analyte is extracted from the sample into the polymeric coating of the fused-silica fiber. After exposure, the fiber is thermally desorbed in the heated injection port of the gas chromatograph, and a chr...

Introduction: Resin composites are an important part of restorative treatment in modern dentistry. There are many trademarks available in Iran market and physico-chemical properties of many of these resin composites are not evaluated yet. This study was carried out to evaluate the physico-chemical properties of four available resin composites and also to assess the effect of aging on their prop...

Accurate detection of organisms is crucial for the effective management of threatened and invasive species because false detections directly affect the implementation of management actions. The use of environmental DNA (eDNA) as a species detection tool is in a rapid development stage; however, concerns about accurate detections using eDNA have been raised. We evaluated the effect of sampled wa...

The determination of radium and/or uranium concentrations in natural waters is useful in a wide range of environmental studies. In this work, a low-background liquid scintillation system (Wallac, Quantulus 1220) was used to determine simultaneously 226Ra and uranium (total) activities in natural water samples. Sample preparation involved filtration and 1 : 10 concentration by evaporation. Then,...

this paper gives an account of the implementation of geomorphological, hydrogeological, hydrochemical and isotopic techniques to understand groundwater-surface water interactions in a multiple use area, los padres-la brava corridor (se of buenos aires province, argentina). the isophreatic map reveals a regional flow from the sw to ne sector, and allowed us to define the effluent-influent behavi...

background: workers in wastewater treatment plants are exposed to a wide range of chemicals as well as biological contaminants. objective: to ascertain whether exposure to bio-aerosols under the normal working conditions in wastewater treatment plants is associated with any significant changes in the prevalence of respiratory symptoms and lung function capacities. methods: 198 employees of wast...