INTRODUCTION Diarrhoeagenic Escherichia coli (DEC) are major causes of diarrhoea in Nigeria. This study was conducted to determine the prevalence of diarrhoea caused by DEC within the Federal Capital Territory, Abuja, Nigeria. METHODS A total of 730 rectal swabs obtained from 201 children with diarrhoea and 529 healthy controls aged 0-24 months were cultured for the isolation of Escherichia c...

Enterotoxigenic Bacteroides fragilis (ETBF) is an important part of the human and animal intestinal microbiota and is commonly associated with diarrhea. ETBF strains produce an enterotoxin encoded by the bft gene located in the B. fragilis pathogenicity island (BfPAI). Non-enterotoxigenic B. fragilis (NTBF) strains lack the BfPAI and usually show two different genetic patterns, II and III, base...

Enterotoxin A production in milk was studied by use of variables of milk quality, initial numbers of enterotoxigenic staphylococci, incubation temperature, and time. In both raw and pasteurized milks having a low total viable count, enterotoxin was detected in minimal incubation times of 6 to 9 hr at 35 C, 9 to 12 hr at 30 C, 18 hr at 25 C, and 36 hr at 20 C, after inoculation with 10(6)Staphyl...

The genes encoding the heat-labile toxin of Escherichia coli were isolated by recombinant DNA methods from enterotoxigenic E. coli recovered from a human and a piglet with diarrhea. With restriction endonucleases, a fine-structure map was made for the toxin genes. Both genes were found to be highly homologous within the toxin-coding DNA, but the surrounding DNA sequences were found to be quite ...

Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhoea in children below 5 years of age in endemic areas, and is a primary cause of diarrhoea in travellers visiting developing countries. Epidemiological analysis of E. coli pathovars is traditionally carried out based on the results of serotyping. However, genomic analysis of a global ETEC collection of 362 isolates taken from pat...

Growth of human, but not porcine enterotoxigenic Escherichia coli (ETEC) isolates is inhibited during incubation with porcine intestinal epithelial cells and by a constitutively produced factor(s) present in unstimulated cell supernatants. The inhibitory factor(s) is heat stable, not produced by serum-starved cells, and is present in a diverse number of cultured epithelial cell lines of animal,...

A hospital stool survey of Indonesian children less than 5 years of age determined the prevalence of diarrhea caused by enterotoxigenic Escherichia coli (ETEC) and other bacterial enteropathogens, compared to non-diarrheic control patients. ETEC were the second most frequent cause of diarrhea, isolated from 16 of 194 (8.2%) of patient's stools compared to 2 of 97 (2.1%) of control stools. The h...

millions of diarrheal disease is made by enterotoxigenic e. coli (etec) each year, specifically in developing countries. in the pathogenesis of etec infections, the first phase is sticking of the bacterium to the minute intestinal epithelium, as a result of colonization factors (cfs) mediation and subsequently generate enterotoxins. these cfs in accordance with their structure are diverged into...

Background: Staphylococcal food poisoning (SFP) is one of the most common food-borne diseases worldwide resulting from contamination by preformed S. aureus enterotoxins and milk considered to be a major source SFP. This study aimed at PCR based detection its enterotoxigenic genes in raw products. Methods: A total 300 samples products were collected randomly Aizawl district Mizoram subjected phe...

Oral administration of vaccines is simpler and more acceptable than injection via needle and syringe, particularly for infants (Fig. 1) This route is promising for new vaccines in development against enterotoxigenic Escherichia coli (ETEC) and Shigella that cause childhood diarrhea with devastating consequences in low-resource countries. However, vaccine antigens and adjuvants given orally need...