In this study, 210 intestinal Escherichia (E.) coli isolates from enteropathogenic diseased pigs were screened for their virulence factors (F4, F5, F6, F18, STa, STb, LT, STx2e and intimin) by PCR and serogrouped by slide-agglutination (O8:K87, O45, O138:K81, O139:K82, O141:K85, O147:K89, O157 and O149:K91). In total, 24.8% enterotoxigenic E. coli, 7.1% enteropathogenic E. coli and 5.2% oedema ...

A bacteriological study was done on the stool of 240 infants and young children. Ninety nine (41 2%) cases were found to be positive for causative agents of gastroenteritis. Stool cultures were positive for bacteria in 40% and for parasite in 1.3% patients. Thirty per cent of the cases had enteropathogenic E. coli (EPEC), followed by Proteus morganii (47%), Shigella (2.0%), Salmonella (1-6%), P...

A total of 220 enteroadherent Escherichia coli were identified from 729 Egyptian children with diarrhea using the HEp-2 adherence assay. Enteropathogenic E.coli (EPEC = 38) was common among children <6 months old and provoked vomiting, while diffuse-adhering E.coli (DAEC = 109) induced diarrheal episodes of short duration, and enteroaggregative E.coli (EAEC = 73) induced mild non-persistent dia...

Address: 1Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil, Rua Botucatu, 862, 3° andar, Vila Clementino, São Paulo, São Paulo, CEP 04023-062, Brazil, 2Institut für Infektiologie, Zentrum für Molekularbiologie der Entzündung, Westfälische Wilhelms-Universität, Münster, Germany, 3E. coli Reference Laboratory (LREC), Department of Microbiology and Parasitology, Faculty of Veterinary...

To assess public health risks from environmental exposure to Extended-Spectrum β-Lactamases (ESBL)-producing bacteria, it is necessary to have insight in the proportion of relative harmless commensal variants and potentially pathogenic ones (which may directly cause disease). In the current study, 170 ESBL-producing E. coli from Dutch wastewater (n = 82) and surface water (n = 88) were characte...

Serogroup O80 was detected in 40% of 104 enteropathogenic Escherichia coli isolates from calves with diarrhea from 42 farms in Belgium during 2008‒2015. These isolates harbored the eae-ξ and fliCH2 genes, similar to the O80 attaching-effacing Shigatoxigenic E. coli isolates found in humans in France. This strain might be emerging.

The penicillin-binding proteins of 11 pathogenic Escherichia coli strains, including enteropathogenic, enterotoxigenic, enteroinvasive, enteroaggregative, and enterohemorrhagic E. coli, were detected in gels following the labeling of isolated cell envelopes with [3H]benzylpenicillin. The electrophoretic profiles, sensitivities to and morphological changes induced by beta-lactam antibiotics show...

The presence of the pathogenicity island (PAI) O122 genes, efa1 (lifA), sen, pagC, nleB, and nleE, in typical and atypical enteropathogenic Escherichia coli (EPEC) strains was investigated. The simultaneous occurrence of all genes was statistically associated with diarrhea due to atypical EPEC. Detection of the complete PAI O122 could aid in the identification of potential pathogenic strains of...

We describe the characterization of 126 atypical enteropathogenic Escherichia coli (aEPEC) isolates from 1,749 Brazilian children. Classic aEPEC strains were more frequently found in children with diarrhea than in controls (P < 0.001), showing their importance as acute diarrhea agents in our country. Only aEPEC strains carrying either the ehxA or paa gene were significantly associated with diar...