We determined the antibiotic susceptibilities of 1,785 enterococcal bloodstream isolates collected over 25 years. Antibiotic resistance emerged at a greater rate in Enterococcus faecium than in other enterococcal species, and E. faecium isolates became proportionally more common over time. Our findings confirm the pattern of emerging antibiotic resistance among enterococci and highlight the inc...

A collection of 250 enterococci isolated from various food-stuffs were used to investigate seven virulence determinants and the microbial susceptibility of eight antibiotics. Species-specific PCR revealed the presence of E. faecalis (127 isolates), E. faecium (77 isolates), E. casseliflavus (21 isolates), E. mundtii (19 isolates) and E. durans (six isolates). Multiplex PCR for virulence factors...

The distribution characteristics of vancomycin-resistant enterococci (VRE) and the resistance of enterococcus isolates to various antibiotics were investigated in Yae River, which flows through Miyazaki city, Japan. The prevalence of VRE among specimens collected from the urban river basin using mEI agar was 0.9% (2 of 226 enterococcal isolates). In the 333 enterococcal isolates obtained using ...

MDL 62,879 (GE2270 A) is a new peptide antibiotic that inhibits protein synthesis through an interaction with elongation factor Tu. MDL 62,879 was very active against gram-positive clinical isolates, particularly staphylococci and enterococci, for which MICs for 90% of isolates were < or = 0.13 micrograms/ml. It was equally active against isolates resistant to beta-lactams, erythromycin, gentam...

This study aimed to isolate Enterococcus sp. from bovine rumen samples. Enterococci are the dominant bacteria in the rumen. Isolation were carried out using classical method by using Streptococcus selective medium then the isolates were identified using modern techniques by real time PCR, gel electrophoresis and DNA sequencing techniques. Results showed that Enterococcus sp. enumerated in the r...

A set of reference strains and a group of previously unidentified enterococci were analysed by rep-PCR with the (GTG)(5) primer to evaluate the discriminatory power and suitability of this method for typing and identification of enterococcal species. A total of 49 strains representing all validly described species were obtained from bacterial collections. For more extensive evaluation of this i...

Neomycin blood agar is commonly used as a selective medium for the isolation of vancomycin resistant enterococci from faeces; however, not all isolates are recovered using this medium, perhaps because the neomycin concentrations are too high. To test this hypothesis, the neomycin minimum inhibitory concentration (MIC) was determined for 27 vancomycin resistant Enterococcus faecium isolates, 14 ...

Six beta-lactamase-producing (Bla+) isolates of Enterococcus faecalis recovered over a 17-month period from an Argentinian pediatric hospital were found to have identical or almost identical chromosomal restriction patterns by pulsed-field gel electrophoresis, although the plasmid patterns were different. These isolates, like Bla+ enterococci in the United States, hybridized to a staphylococcal...

1. Waren, D. K., Kollef, M. H., Seiler, S. M. et al. (2003). The epidemiology of vancomycin-resistant Enterococcus colonization in a medical intensive care unit. Infection Control and Hospital Epidemiology 24, 257–63. 2. Bíliková, E., Hanzen, J., Roidová, A. et al. (2001). Bacteraemia due to vancomycin-resistant enterococci in Slovakia. Journal of Antimicrobial Chemotherapy 47, 357–68. 3. Dutka...