Enteric protozoan parasites, which are spread by the fecal-oral route, are important causes of diarrhea (Giardia duodenalis) and amebic dysentery (Entamoeba histolytica). Cyst walls of Giardia and Entamoeba have a single layer composed of fibrils of β-1,3-linked GalNAc and β-1,4-linked GlcNAc (chitin), respectively. The goal here was to determine whether hand sanitizers that contain ethanol or ...

AIM To explore the prevalence of amebiasis in inflammatory bowel disease (IBD), Crohn's disease and ulcerative colitis, in patients in Clinical hospital Mostar (Bosnia and Herzegovina, region of Herzegovina). METHODS In this study, Entamoeba histolytica/dispar prevalence was investigated in fresh faeces by native microscopy and immunochromatographic rapid assay "RIDA(®)QUICK Entamoeba test", ...

Table. Sequencing outcomes for remaining 13 of 15 samples that were negative by PCR for Entamoeba histolytica, E. dispar, and E. moshkovskii and that were not the novel species E. bangladeshi Specimen no. Sequencing outcome 1 97% identity to E. bangladeshi, but insufficient sequence length led to ambiguous assignment, conservatively removed from further analysis 2 No priming upon sequencing 3 N...

Entamoeba histolytica is a protozoan parasite that causes amebic dysentery and liver abscess. The disease is common in tropical regions of the world where hygiene and sanitation is often approximate. The epidemiology of E. histolytica has been studied around the world. However, there is a dearth of comprehensive literature on the epidemiology of this pathogen as well as its pathogenicity in the...

Entamoeba histolytica infection is the third-greatest parasitic disease responsible for death in the world. Amoebic infections result either in a harmless colonization of the intestine, or in an amoebiosis with invasion and damage of the intestine, liver, lung, and brain. These distinct manifestations are due to the existence of Entamoeba histolytica alone or with Entamoeba dispar as a complex ...

Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced c...

In recent years, substantial progress has been made in understanding the molecular and cell biology of the human parasite Entamoeba histolytica, an important pathogen with significant global impact. This review outlines some recent advances in the Entamoeba field in the last five years, focusing on areas that have not recently been discussed in detail: (i) molecular mechanisms regulating parasi...

We compared the performance of the BD Max enteric parasite panel to routine microscopy and an in-house PCR for the detection of Giardia intestinalis, Entamoeba histolytica, and Cryptosporidium spp. The enteric parasite panel showed good specificity for all targets and good sensitivity for E. histolytica and Cryptosporidium spp. Sensitivity for G. intestinalis with the BD Max enteric parasite pa...

The causative agent of amoebiasis is currently attributed to two distinct species (E. histolytica and E. dispar). The aim of this study was to differentiate these species by PCR in stool samples. Isolated genomic DNA was amplified by PCR and band products of 101 bp (E. dispar) were obtained. All seven stool samples were found to be E. dispar, not E. histolytica. Our results demonstrated the sig...

A comparison of the use of three commercially available enzyme-linked immunosorbent assay-based kits and PCR amplification of rRNA genes to detect and differentiate Entamoeba histolytica from E. dispar was carried out. Only the Techlab kit did not cross-react with E. dispar antigens, but it was 100 times less sensitive than PCR in detection of and differentiation between the two types of Entamo...