نتایج جستجو برای: elisa kit
تعداد نتایج: 72091 فیلتر نتایج به سال:
an indirect enzyme–linked immunosorbent assay (elisa) was developed for screening of antibody to avian infectious bronchitis virus (ibv). antigen was prepared from whole-purified ibv massachusetts serotype (br 801 strain). optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed elisa was determined 0.1μg/ml, 1:3000 and 1:100, respe...
Using solid-phase immune electron microscopy (SPIEM) as a reference test, we examined 151 stool specimens from infants and young children with acute gastroenteritis for rotavirus detection by a one-step commercial enzyme-linked immunosorbent assay (ELISA) with labeled monoclonal antibody. Of the 83 samples determined to be positive for rotavirus by SPIEM, 82 were detected as positive by the mon...
A new, sensitive ELISA for human leptin in plasma and cerebrospinal fluid (CSF) was developed, using monoclonal antibodies. The lower limit of detection of this ELISA was 0.78 pg/assay. Both intra- and interassay imprecision values were <7%. The dilution curves of plasma and CSF showed good linearity, and the recovery was 83.2-95.6%. There was good correlation between plasma leptin concentratio...
Background: Cystic echinococcosis (CE), as a chronic parasitic disease, is a major health problem in many countries. The performance of the currently available serodiagnostic tests for the diagnosis of CE is unsatisfactory. Objective: The current study aimed at sub-cloning a gene, encoding the B8/1 subunit of antigen B (AgB) from Echinococcus granulosus, using gene optimization for the immunodi...
A chemiluminescence enzyme immunoassay based on magnetic microparticles (MmPs-CLEIA) was developed to evaluate serum α-fetoprotein (AFP) in parallel with traditional colorimetric enzyme-linked immunosorbent assay (ELISA). A systematic comparison between the MmPs-CLEIA and colorimetric ELISA concluded that the MPs-CLEIA exhibited fewer dosages of immunoreagents, less total assay time, and better...
Chlamydia trachomatis is one of the main causes of Sexually Transmitted Diseases (STDs) such as prostatitis and epididymitis in men and cervicitis, endometriosis, vaginitis and ureogenital tract infections in women. Serological tests with sensitivities related to specific antigens are commonly used as routine laboratory tests for diagnosis of Chlamydia. In this research the Chlamydia Major O...
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