An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of IgG serum antibodies to swine Sendai virus. To evaluate the preliminary potential of ELISA, sera from 10 healthy pigs and 34 sera from finishing pigs suffering from respiratory signs were tested. The results were compared with those obtained from the same sera by hemagglutination inhibition assay.

A highly sensitive immuno-PCR method specific for the detection of antigens of and antibodies to bovine herpesvirus 1 was established. This assay attained a high sensitivity of up to 10(7.0) times higher than that of enzyme-linked immunosorbent assay (ELISA) or PCR for antigen detection and 10(5.0) times higher than that of ELISA for antibody detection.

BackgroundThrombotic thrombocytopenic purpura (TTP) is a rare but potentially fatal disorder caused by ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 motif, member 13) deficiency. Prompt identification/exclusion of TTP can thus be facilitated rapid testing. The most commonly utilized (enzyme?linked immunosorbent assay [ELISA]–based) takes several hours to perform so do...

an indirect enzyme–linked immunosorbent assay (elisa) was developed for screening of antibody to avian infectious bronchitis virus (ibv). antigen was prepared from whole-purified ibv massachusetts serotype (br 801 strain). optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed elisa was determined 0.1μg/ml, 1:3000 and 1:100, respe...