1. Respiration and fermentation of yeast receiving 90,000 r of 250 kv. x-rays were studied under a variety of conditions. This dose will nearly completely inhibit growth or colony formation. 2. The apparent effects of irradiation are quite dependent on the K(+) and H(+) of the suspending medium. At pH 4.5 stimulatory effects were observed in KH(2)PO(2) buffer and inhibition in potassium-free (T...

After epididymal maturation, sperm capacitation, which encompasses a complex series of molecular events, endows the sperm with the ability to fertilize an egg. This process can be mimicked in vitro in defined media, the composition of which is based on the electrolyte concentration of the oviductal fluid. It is well established that capacitation requires Na(+), HCO(3)(-), Ca(2+), and a choleste...

A hydrated deep eutectic electrolyte with a water-deficient solvation structure and reduced free water in bulk solution is proposed, resulting highly reversible stable Zn anodes.

Researchers add serum to a classical medium at concentrations of 5 to 10% (v/v) to grow cellsin-vitro culture media. Unfortunately, serum is a poorly defined culture medium componentas its composition can vary considerably while serum-free cell culture media are an excellentalternative to standard serum-containing media and offer several major advantages. Advantagesof us...

Development of serum-free suspension cell culture processes is very important for influenza vaccine production. Previously, we developed a MDCK suspension cell line in a serum-free medium. In the present study, the growth kinetics of suspension MDCK cells and influenza virus production in the serum-free medium were investigated, in comparison with those of adherent MDCK cells in both serum-cont...

In modern technology, the main advantages of aluminum alloys as structural materials are lightness, pliability to stamping, corrosion resistance (in air, is instantly covered with a strong film Al2O3, which prevents its further oxidation), high thermal conductivity, and non-toxicity compounds. These properties have made raw material in aviation aerospace industries and, more recently, composite...

Transverse thin cell layer sections excised from in vitro scales of Lilium ledebourii were cultured on Murashige and Skoog (MS) medium supplemented with various plant growth regulators (PGRs) at different concentrations. Although, bulblets were produced on PGRfree MS medium during organogenesis, addition of 0.25 mg l-1 6-benzyladenine or 5.0 mg l-1 indole-3-acetic acid to the medium increased t...

It is shown that cherry stones-wastes can be recycled as activated carbons for electrode material in supercapacitors. KOH-activation of this precursor at 800-900oC is an efficient process to obtain carbons with large specific surface areas (1100-1300 mg), average pore sizes around 0.9 to 1.3 nm, which makes them accessible to electrolyte ions, and conductivities between 1 and 2 S cm. These feat...

A Li-ion cell typically consists of a carbon-based negative electrode (NE); a porous polymer membrane separator (polypropylene and/or polyethylene); and a lithiated transition metal oxide (LiMxOy, M=Co, Ni, and/or Mn) positive electrode (PE). Electrodes are made by casting slurries of PE or NE-materials, PVDF-binder and carbon-black onto metal current collectors. Mixtures of Li-salts and organi...

Four chromogenic urine culture media were compared to culture on blood agar, MacConkey agar, and CLED (cysteine-, lactose-, and electrolyte-deficient) agar for detection of uropathogens in 1,200 urine specimens. After 2 nights of incubation, 96% of all isolates were recovered on blood agar, 96% were recovered on CLED agar, 92% were recovered on CPS ID2, 96% were recovered on CHROMagar Orientati...