A simple and disposable electrochemical immunosensor was developed for sensitive and selective detection of a protein biomarker via target-induced proximity hybridization and electrochemical stripping analysis of silver nanoparticles (AgNPs). The immunosensor was prepared by assembling single-stranded DNA modified gold nanoparticles (ssDNA@AuNPs) on a graphene oxide modified disposable screen-p...

EXPERIMENTAL SECTION Materials and reagents Tetrabutyl titanate (TBOT), sodium dodecyl sulfate (SDS), sodium dodecyl benzene sulfonate (SDBS) and phosphoric acid (H3PO4) were from Nanjing Chemical Reagent Co., Ltd. (Nanjing, China). Human IgG (HIgG) (Ag), rabbit anti-human IgG (Ab1), and goat anti-human IgG (Ab2) were purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China)....

The technlque of scannlng electrochemical mlcroscopy (SECM) Is descrlbed. I n thls technlque the electrolysls current that flows as an ultramicroelectrode tlp (diameter ca. 10 gm) Immersed In a soiutlon Is moved above a substrate surface is used to characterize processes and structural features of the substrate. Modes of Operation consldered Include collectlon modes, where products electrogener...

background and aims: some nanoparticles can be used in immunoassays to increase sensitivity. this study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (bsa nps). materials and methods: at first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. then the concentration of β-subunit of human chorionic gonadotropin (βhcg) i...

there are increasing evidences that show many xenobiotic chemicals (called as endocrine disruptorchemicals edcs) through interfering with endocrine system, have the capability to induce developmentaland reproductive abnormalities in humans and animals. the yolk protein precursor vitellogenin (vtg) hasproved to be a simple and sensitive biomarker for assessing exposure of fish to edcs especially...

reply to the letter sent by prof. viroj wiwanitkit entitled “radiofrequency radiation and human ferritin”   authors of the article entitled “effects of radiofrequency radiation on human ferritin: an in-vitro enzymun assay” [1] would like to express their satisfactions, since the manuscript have been accessed and used as an element of reflection by a renowned researchers such as prof. wiwanitkit...

in this study, five carp pituitary glands were collected and dispersed enzymatically and mechanically.then, the cells were cultivated as monolayer in mem (minimum essential medium eagle). the culture mediawere collected after 72 h and frozen at -20°c. carp ovarian follicles also were separated mechanically andincubated in bss (basic salt solution) cortland medium in 24-well microplates for 48 h...