A two-step chromatographic procedure was developed for the isolation and purification of hen IgY antibodies from egg yolk. The antibodies were completely separated from vitellin and lipids by hydrophobic interaction chromatography followed by gel filtration. Almost no residual yolk proteins, no immunoglobulin aggregates, and no antibody fragments could be detected in the final extract. Moreover...

The chemical changes occurring during embryonic development have been studied by several workers (l-3). In the hen’s egg there is very little nucleic acid present, whereas a considerable synthesis of both desoxypentose nucleic acid (DNA) and ribose nucleic acid (RNA) occurs in the embryo (4-6). Recently, methods for the determination of purines and pyrimidines in small amounts of tissue have be...

monetary compensation for human eggs used in research is a controversial issue and raises major concerns about women’s health and rights, including the potential of exploitation and undue inducement. human eggs are needed for various types of studies and without payment, it would be impossible to procure sufficient eggs for vital research. therefore, a solution seems necessary to prevent exploi...

this experiment was conducted to evaluate the effects of different levels of portulaca oleracea (po),medicinal plant, on performance, egg traits, blood biochemical and immunity parameters of laying hens. one hundred eighty hi-line (w-36 strain) mature laying hens 65 up to 76 weeks of age were allotted in a completely randomized design consisting of 5 treatments and 3 replicates (12 birds per re...

fifter the egg of a higher organism A is ferti l ized it divides into many / I thousands of cells that arrange themselves into the tissue layers of the embryo. Understanding this elaborate process of embryonic development is one of the outstanding and most difficult problems challenging biologists today. The problem is so complex that it must be broken down into smaller problems before investig...

The objective of this experiment was to evaluate spermatological parameters and fertilizing ability of short term stored semen in different extenders from endemic Abant trout (Salmo trutta abanticus T,1954). Semen was collected from 30 adult males by the hand stripping method without anesthesia. Having determined the main spermatological properties (volume, motility, movement duration, concentr...

Limpets are common inhabitants of the midlittoral and upper infralittoral zones play an important ecological role in coastal ecosystem. The study aimed to assess growth, meat yield, morphological aspects, condition reproduction features Patella caerulea . About 58% collected Mediterranean limpets were concentrated 25.0-34.9 mm shell length group. average yield ratio was calculated as 39.34%. Me...

Glycerides and phospholipids from the same tissue exhibit appreciable differences in their fatty acid composition (Hilditch, 1956; Futter & Shorland, 1957), but very little is known about the distribution of fatty acids among the individual phospholipids. Progress in working out the pathways of phospholipid synthesis in living cells (Kennedy & Weiss, 1956; Smith, Weiss & Kennedy, 1957) has, how...