A simple experimental system was developed for studying the movement of long-chain fatty acids between multilamellar liposomes and soluble proteins capable of binding fatty acids. Oleic acid was incorporated into multilamellar liposomes containing cholesterol and egg yolk lecithin and incubated with albumin or hepatic fatty acid-binding protein. It was found that the fatty acid transferred from...

The unsaturated fatty acyl residues of egg yolk lecithin are selectively removed when bilayer dispersions of the lipid are exposed to decomposing peroxychromate at pH 7.6 or pH 9.0. Mannitol (50 mM or 100 mM)partially prevents the oxidation of the phospholipid due to decomposing peroxychromate at pH 7.6 and the amount of lipid lost is inversely proportional to the concentration of mannitol. N,N...

In 1850, Theodore Gobley, working in Paris, described a substance, 'lecithine', which he named after the Greek 'lekithos' for egg yolk. Adolph Strecker noted in 1862 that when lecithin from bile was heated, it generated a new nitrogenous chemical that he named 'choline'. Three years later, Oscar Liebreich identified a new substance, 'neurine', in the brain. After a period of confusion, neurine ...

Protoplasts isolated from epicotyls of nonhardened winter rye seedlings were spin-labeled with the N-oxyl-4-4-dimethyloxazolidine derivatives of 5-ketostearic (5NS) and 16-ketostearic (16NS) acids. Spectra of the membrane-bound labels showed motional broadening with a rotational correlation time of 1.5 x 10(-8) second for 5NS and 1.5 x 10(-10) second for 16NS at 0 C. A procedure was developed t...

Our previous studies demonstrated that low-grade tea had some beneficial effects on egg production performance and quality. Extracts derived from high levels of active ingredients. Thus, this study investigated the green water extracts (GTWE) supplementation production, quality nutritional value laying hens. A total 192 hens were randomly allotted to four groups T0, T1, T2 T3, which fed with ba...

n-/3-Hydroxybutyrate dehydrogenase is a lipid-requiring enzyme which has an absolute requirement for lecithin for enzymic activity. We have studied the activation of the purified apodehydrogenase by a number of lecithin analogues with modifications in either the hydrophobic or polar regions of the molecule in order to map the structural specificity for the lecithin molecule. The apodehydrogenas...

The in vivo biosynthesis of lecithin in rats has been studied with the precursors choline-1,2-(14)C, ethanolamine-1,2-(14)C and methionine-CH(3)-(14)C or -CH(3)-(3)H. Lecithin synthesis from choline is rapid in all organs. No sex difference was observed in this pathway. The biosynthesis of lecithin by methylation of phosphatidyl ethanolamine is of quantitative significance in the liver, but not...

When pure individual synthetic lecithins became available for study (Baer and Kates, 1949, 1950), there was immediate interest in their possible use in cardiolipin antigens as substitutes for Pangborn lecithin. Rosenberg (1949) reported briefly on synthetic L-c-(dipalmitoyl)-lecithin in antigens for the Hinton, Kline, Rein-Bossack, VDRL, and Kolmer tests. He found that replacement of Pangborn's...