نتایج جستجو برای: egfp

تعداد نتایج: 4422  

2016
Satoshi Ota Kiyohito Taimatsu Kanoko Yanagi Tomohiro Namiki Rie Ohga Shin-ichi Higashijima Atsuo Kawahara

The CRISPR/Cas9 complex, which is composed of a guide RNA (gRNA) and the Cas9 nuclease, is useful for carrying out genome modifications in various organisms. Recently, the CRISPR/Cas9-mediated locus-specific integration of a reporter, which contains the Mbait sequence targeted using Mbait-gRNA, the hsp70 promoter and the eGFP gene, has allowed the visualization of the target gene expression. Ho...

Journal: :Journal of neuroscience methods 2005
C Jomphe M-J Bourque G D Fortin F St-Gelais H Okano K Kobayashi L-E Trudeau

The physiological and pharmacological properties of dopaminergic neurons in the brain are of major interest. Although much has been learned from cell culture studies, the physiological properties of these neurons remain difficult to study in such models because they are usually in minority and are difficult to distinguish from other non-dopaminergic neurons. Here we have taken advantage of a re...

Journal: :Journal of cell science 2002
Hyun-Hee Kong Thomas D Pollard

We developed a reliable method for transient transfection of Acanthamoeba using Superfect (Qiagen) and a vector with the Acanthamoeba ubiquitin promoter and enhanced green fluorescent protein (EGFP) as the reporter gene. The transfection efficiency was 3% for profilin-I-EGFP and EGFP-myosin-II tail, and less than 0.5% for larger constructs such as full length myosin-II or myosin-IC. Profilin-I-...

Journal: :BMC Biotechnology 2009
So-Jung Kwon Kyuyong Han Suhyun Jung Jong-Eun Lee Seongsoon Park Yong-Pil Cheon Hyunjung Jade Lim

BACKGROUND MPG is a cell-permeable peptide with proven efficiency to deliver macromolecular cargoes into cells. In this work, we examined the efficacy of MPG as an N-terminal tag in a fusion protein to deliver a protein cargo and its mechanism of transduction. RESULTS We examined transduction of MPG-EGFP fusion protein by live imaging, flow cytometry, along with combination of cell biological...

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 2004
Linda S Overstreet Shane T Hentges Viviana F Bumaschny Flavio S J de Souza James L Smart Andrea M Santangelo Malcolm J Low Gary L Westbrook Marcelo Rubinstein

Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in newly born granule cells of the dentate gyrus under the transcriptional control of proopiomelanocort...

2014
Yangwu Fang Xudan Lei Xiang Li Yanan Chen Fei Xu Xizeng Feng Shihui Wei Yuhao Li

Demyelinating diseases consist of a variety of autoimmune conditions in which the myelin sheath is damaged due to genetic and/or environmental factors. During clinical treatment, some patients undergo partial remyelination, especially during the early disease stages. However, the mechanisms that regulate demyelination remain unclear. The myelin structure, myelin formation and myelin-related gen...

2014
Ping Li Christopher Burlak Jose Estrada Peter J. Cowan

Several promoters from different origins have been used to drive transgene expression in pigs with variable results. In this study, we identified porcine ROSA26 promoter located on chromosome 13. Porcine ROSA26 promoter was cloned and its activity was examined using enhanced green fluorescence protein (EGFP) as a reporter. EGFP expression was detected in adult liver derived cells (LDC) by flow ...

Journal: :Infection and immunity 2004
Camilo Cuadros Francisco J Lopez-Hernandez Ana Lucia Dominguez Michael McClelland Joseph Lustgarten

Vaccination is the most efficient prophylaxis against a variety of infectious diseases. New vaccination strategies rely on the incorporation of effective adjuvants, which stimulate the innate immune response and, in turn, activate the adaptive immune response. It is well established that flagellin induces inflammatory responses through the activation of antigen-presenting cells (APCs). In order...

2008
Svetlana V. Harbaugh Molly E. Davidson Yaroslav G. Chushak Nancy Kelley-Loughnane

Riboswitches are a type of natural genetic control element that use untranslated sequence in the RNA to recognize and bind to small molecules that regulate expression of that gene. Creation of synthetic riboswitches to novel ligands depends on the ability to screen for analyte binding sensitivity and specificity. In our work, we have coupled a synthetic riboswitch to an optical reporter assay b...

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