The receptor for Factor VIII/von Willebrand factor (F. VIIIVWF) is readily available on circulating platelets. We have found that the stimulation of platelets with traces of thrombin at concentrations that are generated physiologically (0.008 U-0.05 U/ml) induced concentration-dependent binding of 125I-labeled F. VIIIVWF in a steady-state system. The binding induced by thrombin was specific bec...

A B ST R A CT The receptor for Factor VIII/von Willebrand factor (F. VIIIVwF) is not readily available on circulating platelets. We have found that the stimulation of platelets with traces of thrombin at concentrations that are generated physiologically (0.008 U0.05 U/ml) induced concentration-dependent binding of '251-labeled F. VIIIvwF in a steady-state system. The binding induced by thrombin...

Myosin can be precipitated from soluble fraction under different assay conditions. This paper describes a new method for precipitating myosin V from rat brain soluble fraction. Brains were homogenized in 50 mM imidazole/HCl buffer, pH 8.0, containing 10 mM EDTA/EGTA, 250 mM sucrose, 1 mM DTT and 1 mM benzamidine, centrifuged at 45000 x g for 40 min and the supernatant was frozen at -20 degrees ...

Terbium-sensitized luminescence and its applicability towards the detection of Bacillus spores such as anthrax are of significant interest to research in biodefense and medical diagnostics. Accordingly, we have measured the effects of terbium chelation upon the parameters associated with dipicolinate ligation and spore detection. Namely, the dissociation constants, intrinsic brightness, lumines...

صخلملا Seasonal variation of zooplankton biomass has been carried out at two selected sites on Greater Zab River. Monthly samples of water were collected during period from January to December 2008. Some physico-chemical properties of water were studied including water temperature (from 8 to 30.5 C), hydrogen ion concentration (from 6.3 to 8), EC (from 255 to 821 μs.cm), turbidity (1085 NTU), d...

Lead (Pb), depending upon the reactant surface, pH, redox potential and other factors can bind tightly to the soil with a retention time of many centuries. Soil-metal interactions by sorption, precipitation and complexation processes, and differences between plant species in metal uptake efficiency, transport, and susceptibility make a general prediction of soil metal bioavailability and risks ...

after thawing they were first diluted and washed by centrifugation in the cold with buffered 5 mM-EDTA (pH 7.4), to remove all Mg’+, followed by two washes with 5 mM-Hepes (adjusted to pH 7.4 with NaOH). After resuspension, the membranes were divided into several aliquots, with or without MgClz or other additions. The progress of the ATP[ I HIADP exchange and [y”P]ATPase reactions was then meas...

The aggregation of group A streptococci by whole, stimulated human saliva (WHS) and the effect of saliva on streptococcal adherence to host cells was investigated. WHS samples from 11 individuals were found to aggregate both M+ and M- group A streptococci to various degrees. The aggregating activity was sensitive to heat, EDTA, EGTA [ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraace...

Removal of rabbit psoas strips immediately after death and incubation in a saline solution containing 1 mM Ca(2+) and 5 nM Mg(2+) for 9 hr at 37 degrees C and pH 7.1 causes complete Z-line removal but has no ultrastructurally detectable effect on other parts of the myofibril. Z lines remain ultrastructurally intact if 1 mM 1,2-bis-(2-dicarboxymethylaminoethoxy)-ethane (EGTA) is substituted for ...

خلا صلا ـ ة : عون نم يفيكتلا ةانقلا ردقم ءادلأ اًضرع ثحبلا اذه مدقي LMS ةيئاضفلا ةرفشملا تاعومجملا مظن يف نييئاوه مدختست يتلا ةينمزلا لإل اسر ل لابقتسلال اًدحاو اًيئاوهو . و ةانق يه ةمدختسملا ةيكلسلالا ةانقلا Rayleigh ءاضوضلا دوجوب ىسواجلا (Gaussian) يمجتلا جارخلاا يف يع . و حضوت ثحبلا جئاتن إ دحاو لماعم يذ ةانق ردقم مادختساب زاحنم ريغ ريدقت ىلع لوصحلا ةيناكم ) يراسم نم لكل لإا ةراش .( و وحن ةيف...