The absorption of the nonionic contrast medium iohexol, the clearance tracer 51Cr-ethylenediaminetetraacetic acid, and the blood-pool marker 125I-human serum albumin was studied after subarachnoid and subdural injection in rabbits. Subdural deposition of the contrast medium and 51Cr-ethylenediaminetetraacetic acid resulted in a faster absorption rate and higher achieved blood levels than a suba...

A cross-sectional study was carried out to determine the current status of Brucella antibodies in goats in Kaduna North Senatorial District of Kaduna State, Nigeria. A total of 442 serum samples (31 bucks and 411 does) were screened using Rose Bengal plate test (RBPT), serum agglutination test with ethylene diaminetetraacetic acid (SAT-EDTA), and lateral flow assay (LFA). Results. The prevalenc...

A polysome extract from salivary glands of C. tentans was sedimented in a 15-60% sucrose gradient. Fractions from the heavy polysome region (1,000-2,000S) and fractions from the light polysome region (200-1,000S) were pooled separately, and the long-term labeled RNA was released by Sarkosyl/pronase and analysed by in situ hybridization. The results showed that BR 1 and BR 2 sequences were prese...

Heavy metal contamination of soils has become a serious problem in industrial or agricultural areas. Soils polluted with heavy metals pose a significant health risk to humans as well as plants and animals, often requiring soil remediation practices. Synthetic chelates such as ethylenediaminetetraacetic acid (EDTA) have been shown to enhance phytoextraction of some heavy metals from contaminated...

Colorimetry of iron in serum with Ferrozine (as used in the Technicon SMAC) or with bathophenanthroline (as used in the Du Pont aca) is influenced by EDTA, in contrast to such measurements with atomic absorption spectroscopy. Therefore EDTA contamination should be avoided with these colorimetric methods. If, however, contamination with EDTA is suspected, addition of zinc sulfate to serum or to ...

Abstm&-The celcium content of blood mrum cm be accurately determined on 0.1-0.25 ml samples by atomic absorption measurements in an air-acetylene flame after removal of the proteins by coagulation and centrifuging. A fairly accurate value can be obtained more quickly from direct measurements on serum diluted with water, which may with advantage contain about 1 per cent of the disodium salt of e...

It has been found that under a wide range of physico-chemical conditions a positive correlation exists between the rate of disappearance of hemolytically active, partially purified first component of human complement and the rate of activation of an esterase hydrolyzing N-acetyl-L-tyrosine ethyl ester. Both reactions follow the kinetic equation for second order autocatalysis, with an apparent e...

An open reading frame, encoding for KDOPS (3-deoxy-D-manno-octulosonate 8-phosphate synthase), from Arabidopsis thaliana was cloned into a T7-driven expression vector. The protein was overexpressed in Escherichia coli and purified to homogeneity. Recombinant A. thaliana KDOPS, in solution, displays an apparent molecular mass of 76 kDa and a subunit molecular mass of 31.519 kDa. Unlike previousl...

The application of calcium chelating agents (EDTA or EGTA) to the tips of maize roots caused a loss of gravitropic sensitivity. When the chelator was replaced with calcium chloride, gravitropic sensitivity was restored. Asymmetric application of calcium chloride near the tip of a vertical root caused curvature toward the calcium source. When the calcium was applied to the upper surface of the t...

The growth inhibitory activity of the epidioxide (II), a precursor in the synthesis of abscisic acid (ABA), has been confirmed with additional assay systems. Under physiological conditions the epidioxide is rearranged to give ABA and an isomer of ABA which has probably the structure V. This major product has very low, if any, biological activity. The biological activity of the epidioxide is exp...