to determine the contamination of traditional cheeses to e. coli o157:h7 this study was conducted in chaharmahal and bakhtiari province. two hundred cheeses samples were obtained randomly and bacteriological examinations were done on all isolates, by culture in selective enrichment media and after that, selective plating. suspected colonies were examined with o and h antisera for identification...

shiga toxin-producing escherichia coli (stec) are food-borne pathogens primarily associated with the consumption of contaminated ground beef and are an important food safety concern worldwide. stec has been found to produce a family of related cytotoxins known as shiga toxins (stxs). shiga toxins have been classified into two major classes, stx1 and stx2. a single strains of stec can produce st...

Cellular eicosapentaenoic acid (EPA) makes up approximately 3% of total fatty acids in Escherichia coli DH5α, a strain that carries EPA biosynthesis genes (pEPAΔ1). EPA was increased to 12% of total fatty acids when the host cell coexpressed the vector pGBM3::sa1(vktA), which carried the high-performance catalase gene, vktA. Where this vector was coexpressed, the transformant accumulated a larg...

The pBR322 plasmid contains genes encoding for tetracycline and ampicillin resistance. Escherichia coli DH5α cells with pBR322 plasmid were grown and plated on selective plates containing 1) ampicillin only and 2) tetracycline only with varying antibiotic concentrations of half strength, three-fourth strength and full strength (15 μg/mL tetracycline and 50 μg/mL ampicillin for full strength). C...

In this study, to know about the phylogeny of Escherichia coli isolated from broilers with collibacillosis in Tabriz, 70 E. coli isolates recovered from broilers with collibacillosis were characterized for phylogenetic group (A, B1, B2, D) by multiplex PCR. Of the all 70 samples, 35 (50%) isolates were classified as type A, 32 (45%) as type D, 2 (2.8%) as type B1 and 1 (2.8%) as type B2. This s...

This study was designed to compare the efficacy of a phytobiotic containing a mixture of essential oils of Oreganum aetheroleum and an antibiotic containing ciprofloxacin as an active principle for the treatment of experimental Escherichia coli (E. coli) infection in broiler chickens. Two-hundred-day-old broiler chickens were divided into 5 equal groups. The 1st group was neither challenged nor...

the present study was conducted to detect the occurrence, serotype, genotype, phylogenetic relationship and antimicrobial resistance pattern of stec from healthy goats of west bengal, india. from the 125 faecal samples collected from healthy goats, 245 isolates were identified as escherichia coli. the e. coli harbouring any gene for shiga toxins (stx1/stx2) was detected in 36 (14.7%) of the 245...

background and objectives: diarrhea is one of the most prevalent diseases in the world, specially in developing countries. one of the most important causative agents of bacterial diarrhea is diarrheagenic escherichia coli (dec) which causes gastroenteritis and this group involving enterotoxigenic e. coli (etec), enteropathogenic e. coli (epec), enteroaggregative e. coli (eaec), enterohemoragic ...

conclusions the results of the present study showed that the baculovirus expression system (sf9 cells) was able to express truncated orf2 and truncated orf2-nsp4 proteins as a potential candidate vaccine. background the hepatitis e virus (hev) accounts for hepatitis e infection with relatively high mortality rate in pregnant women that can lead to fulminant hepatitis. the baculovirus expression...

Our long-term goal is to develop a hybrid cellulose-copper nanoparticle material as a functional nanofiller to be incorporated in thermoplastic resins for efficiently improving their antimicrobial properties. In this study, copper nanoparticles were first synthesized through chemical reduction of cupric ions on TEMPO nanofibrillated cellulose (TNFC) template using borohydride as a copper reduci...