Objective: To explore the antibacterial activity of combined use Shuanghuanglian and cefoperazone sulbactam sodium on resistant strains Pseudomonas aeruginosa. Methods: The aeruginosa which were sensitive to selected prepare different test bacterial solutions respectively; experimental liquid Cefoperazone Sulbactam Sodium prepared separately set as groups control groups; Drug Sensitivity Tests ...

In 2013 we published an analysis demonstrating that drug response data and gene-drug associations reported in two independent large-scale pharmacogenomic screens, Genomics of Drug Sensitivity in Cancer (GDSC) and Cancer Cell Line Encyclopedia (CCLE), were inconsistent. The GDSC and CCLE investigators recently reported that their respective studies exhibit reasonable agreement and yield similar ...

Colon cancer is one of the tumors most refractory to treatment by chemotherapy, and this may be due to inherent phenotypic instability of such tumor cells with respect to the biochemical determinants of drug sensitivity. To test this hypothesis, a clonal human colon carcinoma cell line, clone A, was passaged in culture in the absence of selection conditions or mutagens. During this time, sensit...

Aberrant promoter hypermethylation and associated gene silencing are epigenetic hallmarks of tumorigenesis. It has been suggested that aberrant DNA methylation can affect the sensitivity of cancers to antineoplastic agents by altering expression of genes critical to drug response. To study this issue, we used bisulfite PCR to assess DNA methylation of 32 promoter-associated CpG islands in human...

Bloodstream forms of four populations of the livestock pathogen Trypanosoma congolense, isolated from different natural infections, have been shown to exhibit a wide range of sensitivities to the trypanocide isometamidium chloride (Samorin(R)). In mice the 50% curative doses (CD50) for Samorin range from 0.007 to 20 mg/kg body weight. Uptake of isometamidium chloride demonstrated Michaelis-Ment...

The sensitivity of cancer cells to anticancer drugs is a crucial factor for developing effective treatments. However, it is still challenging to precisely predict the effectiveness of therapeutics in humans within a complex genomic and molecular context. We developed an interface which allows the user to rapidly explore drug sensitivity and gene expression associations. Predictions for how expr...

Hypernatremia is defined as plasma Na+ concentration above 145 meq/L (often due to absolute body water loss and not to total sodium excess). Nevertheless, when kidney damage is present, as occurs in renal diseases, hepatic cirrhosis and congestive heart failure, total body sodium can be increased. The present study evaluates the relationship between hypernatremia and response to anesthetic drug...