the human granulocyte-macrophage colony stimulation factor (hgm-csf) gene was cloned in the pet 23a( +) expression vector under the control of strong bacteriophage t7 transcription and translation signals. the hgm-csf gene was transferred into e. coli strainbl21 (de3)plyss andiptg was used for induction of gm-csf gene. production of the target protein was obtained as revealed by elisa and weste...

BACKGROUND & OBJECTIVE Salmonella induced enteritis is a complex phenomenon involving a number of pathogenic factors. Type III secretions play a central role in the development of Salmonella induced enteritis. One such Type III secretion protein is Salmonella outer proteinB (SopB). Reports on the prevalence of this gene among different serovars of Salmonella of Indian origin appears to be lacki...

In order to overcome some of the limitations of conventional microbiological techniques in the diagnosis of human brucellosis, a simple PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed. After amplification of a 223-bp sequence of a gene that codes for the synthesis of an immunogenetic membrane protein specific for the Brucella genus (BCSP31), the digoxigenin-labeled amplified pro...

definitive hosts of the echinococcus granulosus (e. granulosus ) parasite are carnivores such as dogs,wolves and foxes. detection of this parasite through faecal examination is not possible. in this study, dotblotting test for e. granulosus -specific coproantigens has been evaluated in dog. three 2–3-month-old puppies were treated with piperazine and then faecal samples were collected as pre-in...

Polyclonal antibodies of predetermined specificity for pediocin PA-1 (pedA1) have been generated by immunization of rabbits with a chemically synthesized C-terminal fragment of this bacteriocin (PH2) conjugated to the carrier protein keyhole limpet hemocyanin (KLH). The sensitivity and specificity of the PH2-KLH-generated antibodies were evaluated by the development of various enzyme-linked imm...