the present work developed an efficient plant regeneration and propagation system via direct organogenesis for morinda officinalis how., a valuable endangered medicinal plant. the effects of explant types on shoot induction, plant growth regulators on shoot proliferationand elongation and the subsequent rooting ability of shoots were examined. among the various types of explant, direct shoot pr...

Vietnam has planning in re-forest of Chukrasia tabularis, a special-use forest and re-culture Coffea sp., an industrial crops. The first barrier is seedlings for supplying higher demand from farmers. Using thin cell layer culture method one the ways to resolve woody species. Results gave capacities using shoot-tips regeneration them far propagation. tabularis A. Juss: Shoot ti...

dendrobium chrysanthum (orchidaceae) is highly valued in the ornamental industry and has important medicinal properties used in the preparation of herbal medicines in different parts of the world. however, this orchid species is getting depleted from its natural habitats due to unsystematic collections. therefore, in the present study, an efficient mass propagation protocol through direct shoot...

An improved organogenesis protocol for multiplication of sweet orange cv. Mosambi has been standardized by the use different PGRs, basal media and ethylene adsorbents. Incorporation cytokinins, BAP (8.8 µM) kinetin (6.97 resulted in best shoot with highest response (81.40%), no. micro-shoots/explant (2.06), mean micro-shoot length (1.30 cm) leaves/micro-shoot (3.55) owing to complementary effec...

Pittosporum eriocarpum Royle, a medicinally important taxon, is endemic to Uttarakhand region of Himalaya. It has become endangered due to over-collection and the loss of habitats. As raising plants through seeds in this plant is problematic, a reliable protocol for micropropagation using nodal explants has been developed. High shoot regeneration (95%) occurred in MS medium augmented with BA 0....

parsi zira, bunium persicum (boiss.) b. fedtsch., or black zira, a member of the umbellifera family, is one of the most important medicinal species with high economic value. generally, there is limited information about in vitro culture of bunium persicum. in this study, the effect of various media (ms, b5) and combinations of plant growth regulators, naa and 2,4-d alone or together with kin, o...

Plants have a profound capacity to regenerate organs from differentiated somatic tissues, based on which propagating plants in vitro was made possible. Beside its use in biotechnology, in vitro shoot regeneration is also an important system to study de novo organogenesis. Phytohormones and transcription factor WUSCHEL (WUS) play critical roles in this process but whether and how epigenetic modi...

An efficient protocol was established for rapid in vitro propagation of Stevia rebaudiana Bertoni, using explants from mature plants. The effect of 1/2MS, MS and B5 culture media, also 27 different combinations of auxins and cytokinins was studied on in vitro growth and development of Stevia rebaudiana Bertoni. The best culture medium for shoot and root formation was 1/2MS, producing 1.30 shoot...

Three theories of apical dominance, direct, diversion, and indirect, were proposed in the 1930s to explain how auxin synthesized in the shoot apex might inhibit axillary bud outgrowth, and thus shoot branching. The direct and diversion theories of apical dominance have been investigated in detail, and they are replaced with the current auxin transport canalization and second messenger theories,...

abstract in vitro leaf explants of gf677 hybrid rootstock and rabi cultivar were placed on mql medium supplemented with bap at 0, 1, 2, 3 and 4 mg/l and naa at 0, 0.15 and 0.3 mg/l concentrations. the results revealed that bap, naa, genotype and their interactions had significant effect on both percentage and mean number of the regenerated shoots. for gf677, the highest percentage of shoot rege...