A polymerase chain reaction-restriction fragment mass polymorphism (PCR-RFMP) assay protocol using PGMY09/11 primers for the detection and identification of human papillomavirus (HPV) has recently been developed. The present study evaluated the analytical sensitivity and clinical utility of HPV genotyping employing PCR-RFMP as compared to direct sequencing. Serial dilutions of cloned HPV DNA we...

This paper points out how the nonlinearities involved in multivariable Takagi–Sugeno (T–S) fuzzy control systems could originate complex behavior phenomena, such as multiple equilibrium points or limit cycles, that cannot be detected using conventional stability analysis techniques. In the paper, the application of MIMO frequency-domain methods to predict the existence of multiple equilibria an...

In the present study, a novel broad-range real-time PCR was developed for the rapid detection of human pathogenic fungi. The assay targets a part of the 28S large-subunit ribosomal RNA (rDNA) gene. We investigated its application for the most important human pathogenic fungal genera, including Aspergillus, Candida, Cryptococcus, Mucor, Penicillium, Pichia, Microsporum, Trichophyton, and Scopula...

The stochastically forced three-dimensional Hindmarsh–Rose model of neural activity is considered. We study the effect of random disturbances in parametric zones where the deterministic model exhibits monoand bistable dynamic regimes with period-adding bifurcations of oscillatory modes. It is shown that in both cases the phenomenon of noise-induced bursting is observed. In the monostable zone, ...

Novel means of fabricating polymeric microfluidic devices are presented. An SU-8 master is applied in two-stage embossing, followed by vaporized organic solvent bonding. The primary master is created by standard photolithography; the inexpensive SU-8 primary master is used in a two-stage process to generate microfeatures in hard polymers. A vaporized solvent bonding technique that readily produ...

OBJECTIVES The goal of this study was to identify the factors responsible for embolic complications of direct current (DC) cardioversion of atrial arrhythmias. BACKGROUND Direct current cardioversion of atrial fibrillation (AF) carries a risk of thromboembolism, which is reduced, but not eliminated, by anticoagulation. The risk of embolism after conversion of atrial flutter is believed to be ...

This paper presents the internet-based remote control of mobile robot. To face unpredictable Internet delays and possible connection rupture, a direct teleoperation architecture with “Speed Limit Module” (SLM) and “Delay Approximator” (DA) is proposed. This direct control architecture guarantees the path error of the robot motion is restricted within the path error tolerance of the application....

Quantum limit of dissipation-free photon-photon interactions at the few photon level is studied in the context of giant Kerr nonlinearities in cavity electromagnetically induced transparency (EIT). It is shown that photon-photon interaction is largest when the cavity contains a single atom. In contrast to conventional nonlinearities, increasing the number of atoms cannot increase the interactio...