A method has been investigated for increasing the degree of in vivo labeling of deoxyribonucleic acid (DNA) achieved with tritiated thymidine, by prior administra tion of 5-fluorouracil. 5-Fluorouracil, in therapeutic doses, produced a doubling of the incorporation of thymidine into DNA in bone marrow, but had a negligible effect on incorporation of DNA in the intestinal mucosa, except under co...

Granulocyte colony-stimulating factor (G-CSF), granulocytemacrophage colony-stimulating factor (GM-CSF), ?-interferon (?-IFN), or tumor necrosis factor-a (TNF-a) triggered the rapid, stable phosphorylation of a 75-Kd protein ( ~ 7 5 ) when incubated with permeabilized HL60 human myeloid leukemia cells in the presence of [?-32P] ATP. Among several chemical inducers of HL60 cell differentiation. ...

Fluorine-18 is the most frequently used radioisotope in positron emission tomography (PET) radiopharmaceuticals in both clinical and preclinical research. Its physical and nuclear characteristics (97% β(+) decay, 109.7 min half-life, 635 keV positron energy), along with high specific activity and ease of large scale production, make it an attractive nuclide for radiochemical labeling and molecu...

Although there have been many studies on the prosodic structure of spoken Mandarin as well as many proposals for labeling the prosody of spoken Mandarin, the labeling of prosodic boundaries in all the existing annotation systems relies on auditory perception, and lacks a direct relation to the acoustic process of prosody generation. Besides, perception-based annotation cannot ensure a high degr...

In this study, a remarkably simple and direct strategy has been successfully developed to selectively label target cysteine residues in fully unprotected peptides and proteins. The strategy is based on the reaction between allenamides and the cysteine thiol, and proceeds swiftly in aqueous medium with excellent selectivity and quantitative conversion, thus forming a stable and irreversible conj...

Apoptosis induced in different cell lines can be detected and quantified in one step. Direct labeling of apoptotic cells (DLAC) was performed by incorporation of fluorescein-dUTP (F-dUTP) in DNA strand breaks by terminal deoxynucleotidyl transferase (TdT). Nick-end-labeling using F-dUTP obviates the need for second-step revelation reagents without reducing the specificity of detection. Cells we...

MicroRNAs (miRNAs) are a class of small non-coding RNAs (19 – 25 nucleotides) that regulate gene expression in viruses, plants, and animals. Over 700 miRNAs are already identified in humans with thousands more expected to be found. Accumulated studies have reported distinct miRNA expression patterns in various human diseases, showing great potential of miRNA profiling in clinical applications s...

We describe the first enzyme-driven technique for fluorescent labeling of single-walled carbon nanotubes (SWNTs). The labeling was performed via enzymatic biotinylation of nanotubes in the tyramide-horseradish peroxidase (HRP) reaction. Both direct and indirect fuorescent labeling of SWNTs was achieved using either biotinyl tyramide or fluorescently tagged tyramides. Biotinylated SWNTs later re...

  Introduction: The field of stem cell biology and regenerative medicine is rapidly moving toward translation to clinical practice. Stem cell therapy seems to be a new treatment option for some diseases. So, tracking the distribution of stem cells is crucial to their therapeutic use. Based on this fact we labeled human mesenchymal stem cells (MSCs) with 111...

Let G(V,E) be a graph with p vertices and q edges. A graph G is said to have an odd mean labeling if there exists a function f : V (G) → {0, 1, 2,...,2q - 1} satisfying f is 1 - 1 and the induced map f* : E(G) → {1, 3, 5,...,2q - 1} defined by f*(uv) = (f(u) + f(v))/2 if f(u) + f(v) is evenf*(uv) = (f(u) + f(v) + 1)/2 if f(u) + f(v) is odd is a bijection. A graph that admits an odd mean labelin...