A novel co-digestion approach was evaluated to determine if agricultural and industrial waste residues could efficiently degrade straw without pretreatment. Untreated rice straw was co-digested with pig wastewater and anaerobic sludge from the pulp and paper mill treatment process in a pilot-scale digester (1 m) operated in dry, mesophilic conditions. The total weight ratio of dry straw to pig ...

The glycaemic index (GI) characterises foods by using the incremental area under the glycaemic response curve relative to the same amount of oral glucose. Its ability to differentiate between curves of different shapes, the peak response and other aspects of the glycaemic response is contentious. The present pilot study aimed to explore the possibility of using H NMR spectroscopy to better unde...

Background and Aims: In this study we improved the purification of immunoglobulins from equine antiserum raised against the venom of 6 types of scorption species. Caprylic acid (octanoic acid), a fatty acid, was found to have no effect on the activity of the enzymes pepsin, which is used in antivenom purification to digest Fc fragment of immunoglobulins to obtain F(ab´)2. Materials and Methods...

Atomic force microscopy (AFM) allows the analysis of individual polymers at nanostructural level with a minimal sample preparation. This technique has been used to analyse the pectin disassembly process during the ripening and postharvest storage of several fleshy fruits. In general, pectins analysed by AFM are usually visualized as isolated chains, unbranched or with a low number of branchs an...

Comprehensive characterization of physicochemical properties monoclonal antibodies (mAbs) is a critical process to ensure their quality, efficacy, and safety. For this purpose, mAb analysis at different levels (bottom-up, middle-up) common approach that includes rather complex multistep sample preparation (reduction, digestion). To high performance, the development fully integrated methodologie...

In recent years many researchers show a high interest in co-digestion, simultaneous anaerobic decomposition of a homogenous mixture of at least two biodegradable waste. Anaerobic codigestion is reported to offer several benefits over digestion of separate materials, such as increased cost-efficiency, increased biodegradation of the treated materials, as well as increased biogas production. Most...

Abstract The biomass valorisation of the invasive brown alga Rugulopteryx okamurae (Dictyotales, Phaeophyceae) is key to curbing expansion this macroalga which generating tonnes on southern Spain beaches. As a feasible alternative for management, anaerobic co-digestion proposed in study. Although digestion macroalgae barely produced 177 mL CH 4 g −1 VS, with C-rich substrate, such as olive mill...

in this work a rapid and selective procedure for separation and preconcentration of lead(pb2+) before determination by electrothermal atomic absorption spectrometry (etaas) wasdeveloped. the procedure is based on the electrodeposition on a graphite probe modified withpalladium. the lead was deposited from acetate buffer solution at ph 5.5. various parameters,such as ph of solutions, deposition ...

A digestion trial using cannulated lactating cows was conducted to evaluate the influence of barley grain processing on characteristics of ruminal fermentation and the site and extent of digestion. The experiment consisted in 4 periods and lasted 84 days. The total mixed rations contained 39.86% of grains and 42.90% of alfalfa hay and the rest of ration was composed mainly by cane molasses, fat...

The formation of drug-protein adducts in vivo may have important clinical and toxicological implications. Consequently, there is a great interest in the detection of these adducts and the elucidation of their role in the processes leading to adverse and idiosyncratic drug reactions. Enzymatic digestion is a crucial step in bottom-up proteomics strategies for the analysis of drug-protein adducts...