Differential display has been developed as a tool to detect and characterize altered gene expression in eukaryotic cells. The basic principle is to systematically amplify messenger RNAs and then distribute their 3' termini on a denaturing polyacrylamide gel. Here we provide methodological details and examine in depth the specificity, sensitivity and reproducibility of the method. We show that t...

Phaseolus vulgaris phytohemagglutinin L is a homotetrameric-leucoagglutinating seed lectin. Its three-dimensional structure shows similarity with other members of the legume lectin family. The tetrameric form of this lectin is pH dependent. Gel filtration results showed that the protein exists in its dimeric state at pH 2.5 and as a tetramer at pH 7.2. Contrary to earlier reports on legume lect...

We have seen that additive noise technique is not (SV (γ), ε)-DP. So, the question that we explore in todays class is whether differential privacy (DP) is achievable with SV sources. Interestingly, we give a differential private mechanism for approximate arbitrary “low sensitive” functions that works even with randomness coming from SV source, for any γ < 1. We conclude todays lecture with some...

Differential scanning calorimetry (DSC) analysis of ring opening methatesis polymerization (ROMP) derived polydicyclopentadiene (PDCPD) revealed an unexpected thermal behavior. A recurring exothermic signal can be observed in the DSC analysis after an elapsed time period. This exothermic signal was found to be proportional to the resting period and was accompanied by a constant increase in the ...

Differential scanning calorimetry (DSC) was applied to elucidate the thermal behavior of fowl feather keratins (barbs, rachis, and calamus) with different morphological features. The DSC curves exhibited a clear and relatively large endothermic peak at about 110-160 degrees C in the wet condition. A considerable decrease in transition temperature with urea and its helical structure content esti...

Differential scanning calorimetry (DSC) is an analytical technique that measures the molar heat capacity of samples as a function of temperature. In the case of protein samples, DSC profiles provide information about thermal stability, and to some extent serves as a structural "fingerprint" that can be used to assess structural conformation. It is performed using a differential scanning calorim...

Differential scanning calorimetry (DSC), thermogravimetry (TG) and Fourier-transform infra-red spectroscopy (FT-IR) analyses were performed to investigate changes in the physico-chemical properties of botryosphaerans, a family of exopolysaccharides (EPS) produced by the fungus Botryosphaeria rhodina MAMB-05 grown on glucose (EPS(GLC)), sucrose (EPS(SUC)) and fructose (EPS(FRU)). A slight endoth...

When an aluminum alloy is solution annealed and afterwards cooled too slowly, an exothermal precipitation reaction occurs. With increasing cooling rate, the precipitation heat decreases. Since the precipitation reaction is relatively fast, a fast cooling rate on the differential scanning calorimeter (DSC) is essential to obtain the critical cooling rate which is the minimum cooling rate at whic...

BACKGROUND. Mastery refers to the degree to which people perceive that they can control factors that influence their life situation, and has been found important for people’s quality of life and well-being. It is thus essential to be able to measure mastery in a valid and reliable way. AIM. This study aimed at using the Rasch measurement model for investigating the psychometric properties of a ...

Differential display technique was applied to study expression of RNA in tumorigenic and nontumorigenic cell variants of avian sarcoma virus transformed hamster cells. Methodical conditions were worked out, which allowed identifying a cDNA fragment of an unknown gene expressed in nontumorigenic cell variant only. Its role in tumor suppression remains to be determined.