We report the annotated genome sequence of Xanthomonas arboricola pv. pruni strain Xap33, isolated from almond leaves showing bacterial spot disease symptoms in Spain. The availability of this genome sequence will aid our understanding of the infection mechanism of this bacterium as well as its relationship to other species of the same genus.

the ovipositiomil behaviour and success of anagyrus pseudococci (girault), an encyrtid endoparasitoid of mealybugs, planococcus citri (risso) and pseudococcus affinis (mask.) were studied in the laboratory. behavioural sequences during oviposition starting from the searching for the host to the post-oviposition activities were described. once encountered, the mealybug host was examined and ...

xanthomonas citri subsp citri (xcc) is responsible for one of the most destructive diseases of citrus fruits, asiatic citrus canker. based on the bacterial host range, the pathogenic forms, a, a*, and aw have been identified. a*-type strains are not well characterized and they have been reported to differ from other a-type strains on the basis of biochemical and genetic characteristics. in this...

Droplet digital polymerase chain reaction (ddPCR) is a novel molecular biology technique providing absolute quantification of target nucleic acids without the need for an external calibrator. Despite its emerging applications in medical diagnosis, there are few reports of its use for the detection of plant pathogens. This work was designed to assess the diagnosis potential of the ddPCR for abso...

The Asian citrus psyllid, Diaphorina citri, transmits a phloem-limited bacterium, Candidatus 'Liberibacter' asiaticus that causes citrus greening disease. Because juvenile hormone (JH) plays an important role in adult and nymphal development, we studied the final steps in JH biosynthesis in D. citri. A putative JH acid methyltransferase ortholog gene (jmtD) and its cognate cDNA were identified ...

Asiatic citrus canker is a major disease worldwide, and its causal agent, Xanthomonas citri pv. citri, is listed as a quarantine organism in many countries. Analysis of the molecular epidemiology of this bacterium is hindered by a lack of molecular typing techniques suitable for surveillance and outbreak investigation. We report a comparative evaluation of three typing techniques, amplified fra...

The Asian citrus psyllid (Diaphorina citri Kuwayama) is the principal vector of ‘Candidatus Liberibacter asiaticus’ (CLas) associated with huanglongbing (HLB), the most serious citrus disease worldwide. New control measures including pesticides are urgently needed to combat HLB, especially to protect young or newly planted citrus trees from CLas-inoculation by vector psyllids. Here, we tested C...

Diaphorina citri Kuwayama (Hemiptera: Psyllidae) carries Candidatus liberibacter spp., the putative causal agents of Huanglongbing. D. citri reproduces and develops only on the flushing shoots of its rutaceous host plants. Here we examined whether D. citri is attracted to host plant odors and a mixture of synthetic terpenes. Tests conducted in a vertically oriented Y-tube olfactometer showed th...