The dihydropyridine receptor (DHPR), normally a voltage-dependent calcium channel, functions in skeletal muscle essentially as a voltage sensor, triggering intracellular calcium release for excitation-contraction coupling. In addition to this fast calcium release, via ryanodine receptor (RYR) channels, depolarization of skeletal myotubes evokes slow calcium waves, unrelated to contraction, that...

We investigated differences between immature and mature hippocampal neurons in their response to deprivation of oxygen and glucose (in vitro ischemia), using intracellular recording techniques from CA1 pyramidal neurons in rat brain slices. The membrane was more depolarized in immature hippocampal CA1 neurons (postnatal day 7, P7) compared with the adult neurons (P140), and the apparent input r...

The role of plasma membrane depolarization as a determinant of the initial phase of insulin secretion was investigated. NMRI mouse islets and beta-cells were used to measure the kinetics of insulin secretion, ATP and ADP content, membrane potential, and cytosolic free Ca(2+) concentration ([Ca(2+)](i)). The depolarization of metabolically intact beta-cells by KCl corresponded closely to the the...

18 Stick insect middle leg (mesothoracic) motoneurons receive tonic excitatory input during front leg 19 stepping on a treadmill. We studied the pharmacology of this excitatory input to the motoneurons 20 during single-legged treadmill walking (in situ). During bath application of drugs restricted to the 21 mesothoracic ganglion activity in motoneurons contralateral to the stepping front leg wa...

Under single scattering conditions, water droplets clouds do not depolarize the backscattered light. However, backscattered light from multiple scattering will be depolarized. The level of depolarization is a function of the droplets size, the cloud extinction coefficient value and profile; it has also an important dependency on the lidar field-of-view (FOV). The use of depolarization informati...

Contracting skeletal muscle increases glucose uptake to sustain energy demand. This is achieved through a gain in GLUT4 at the membrane, but the traffic mechanisms and regulatory signals involved are unknown. Muscle contraction is elicited by membrane depolarization followed by a rise in cytosolic Ca2+ and actomyosin activation, drawing on ATP stores. It is unknown whether one or more of these ...

We have shown previously that BDNF, neurotrophin-3 (NT-3), chlorphenylthio-cAMP (cpt-cAMP) (a permeant cAMP analog), and membrane depolarization promote spiral ganglion neuron (SGN) survival in vitro in an additive manner, depolarization having the greatest efficacy. Expression of both BDNF and of NT-3 is detectable in cultured SGNs after plating in either depolarizing or nondepolarizing medium...

Media made hyperosmotic with sucrose increase the frequency of spontaneously released quanta of transmitter, or miniature excitatory postsynaptic potentials (MEPSPs). In calcium-free medium, depolarization with high potassium reduces the MEPSP frequency, presumably due to calcium efflux in the reversed gradient condition. This effect of depolarization is blocked by cobalt, supporting the above ...

Vascular smooth muscle cell (SMC) contraction is mediated in part by calcium influx through L-type voltage-gated Ca2+ channels (VGCC) and activation of the RhoA/Rho kinase (ROK) signaling cascade. We tested the hypothesis that Ca2+ influx through VGCCs regulates SMC differentiation marker expression and that these effects are dependent on RhoA/ROK signaling. Depolarization-induced activation of...

• Electrical depolarization of the muscle cell membrane lias been generally considered to be an essential part of the physiological excitation of muscle cells which initiates contraction." However, there is direct evidence for electrical inexcitability of the membrane receptors in frog skeletal muscle, and complete depolarization by immersion in isosmotic potassium-rich solution does not abolis...