Bacterial vaccines can reduce carriage rates. Colonization is usually a binary endpoint. Real time quantitative PCR (qPCR) can quantify bacterial DNA in mucosal samples over a wide range. Using culture and single-gene species-specific qPCRs for Streptococcus pneumoniae (lytA), Streptococcus pyogenes (ntpC), Moraxella catarrhalis (ompJ), Haemophilus influenzae (hdp) and Staphylococcus aureus (nu...

BACKGROUND Planning and evaluating malaria control strategies relies on accurate definition of parasite prevalence in the population. A large proportion of asymptomatic parasite infections can only be identified by surveillance with molecular methods, yet these infections also contribute to onward transmission to mosquitoes. The sensitivity of molecular detection by PCR is limited by the abunda...

BACKGROUND Population-based studies conducted in Latin America have shown a high proportion of asymptomatic and submicroscopic malarial infections. Considering efforts aiming at regional elimination, it is important to investigate the role of this asymptomatic reservoir in malaria transmission in peri-urban areas. This study aimed to estimate the prevalence of Plasmodium spp. and gametocyte bur...

Canine infectious respiratory disease complex (CIRDC) is the primary cause of in canine population and caused by a wide array viruses bacterial pathogens with coinfections being common. Since its recognition late 2019, Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has been reported to dogs. Therefore, rapid detection differentiation SARS-CoV-2 from other common viral agents criti...

uniform linear array (ula)-based tapped-delay line (tdl) structure has good performance metrics when the signal sources are located at the middle angles. it offers poor performance when the signal sources are close to the array endfire. in this paper, a new approach is proposed which offers higher performance and desired beamforming on tdl structure when the wideband uncorrelated radio sources ...

A new four elements compact antenna array is presented and discussed to achieve enhanced phase resolution without sacrificing the array output power. This structure inspired by the Ormia Ochracea’s coupled ears. The analogy between this insect acute directional hearing capabilities and the electrically compact antenna array is used to enhance the array sensitivity to direction of arrival estima...

This paper focuses on the comparison of microbial biomass increase (cell culture growth) using field-relevant testing methods and moving away from colony counts. Challenges exist in exploring antimicrobial growth fastidious strains, poorly culturable bacteria bacterial communities environmental interest. Thus, various approaches have been explored to follow that can be efficient surrogates for ...

Alternative splicing of pre-mRNA generates protein diversity. Dysfunction of splicing machinery and expression of specific transcripts has been linked to cancer progression and drug response. Exon microarray technology enables genome-wide quantification of expression levels of the majority of exons and facilitates the discovery of alternative splicing events. Analysis of exon array data is more...