Subcellular fractions of the electric tissue of the main organ of the eel Electrophorus electricus were prepared in sucrose media by differential centrifugation and differential discontinuous gradient centrifugation. The distributions of acetylcholinesterase, cytochrome oxidase, DNA, and protein were determined. The appearance of the fractions was determined by phase contrast microscopy and by ...

Proteinpolysaccharides from bovine embryonic rudiments and calf nasal septa were compared by conventional ultracentrifugation in isotonic salt solutions, equilibrium density gradient centrifugation, and free flow electrophoresis. These methods are all suitable for use with large amounts of material and give reliable materials balances. Fractions obtained by any one method could be isolated and ...

1. The subcellular distribution of transglutaminase was investigated by using the analytical approach of differential and isopycnic centrifugation as applied to three organs of the rat: liver, kidney and lung. After differential centrifugation by the method of de Duve, Pressman, Gianetto, Wattiaux & Appelmans [(1955) Biochem. J. 63, 604-617], transglutaminase is mostly recovered in the unsedime...

Large amounts of 5-hydroxytryptamine (5-HT) in the mammalian duodenum are found mainly in the enterochromaffin cells. Baker (1) was the first to demonstrate that in the enterochromaffin cells of the dog's duodenal mucosa, 5-HT was stored in large granules which were sedimented in the gravitational field used for the sedimentation of mitochondria. By further equilibrium centrifugation in a sucro...

Viruses are extensively studied as pathogens and exploited as molecular tools and therapeutic agents. Existing methods to purify viruses such as gradient ultracentrifugation or chromatography have limitations, for example demand for technical expertise or specialized equipment, high time consumption, and restricted capacity. Our laboratory explores mutations in oncolytic reovirus that could imp...

In this study we present data on the entire population of MUC2 molecules secreted from and within the cell layer of an intestinal cell line. The molecular size distribution of the extracted molecules and their reactivity with two different MUC2 polypeptide antibodies indicated the presence of precursor and mature forms of the mucin. Oligomerized forms of the mucin were found in both the cell la...

PURPOSE The goal of this study was to develop a molecular diagnostic assay to detect circulating breast cancer cells in the peripheral blood for the purpose of staging breast cancer. Our aim was to make available an assay that was not limited by the low concentration of circulating breast cancer cells and the background gene expression that is typically found in peripheral blood. EXPERIMENTAL...