objective(s):multi-epitopic protein vaccines and direction of vaccine delivery to dendritic cells (dcs) are promising approaches for enhancing immune responses against mutable pathogens. escherichia coli is current host for expression of recombinant proteins, and it is important to optimize expression condition. the aim of this study was the optimization of multi-epitopic hiv-1 tat/pol/gag/env ...

Objective(s)  Garlic (Allium sativum) is known as a potent spice and a medicine with broad therapeutic properties ranging from antibacterial to anticancer, and anticoagulant. One of the major purified garlic protein components is the 47 kDa protein. In this study, the effect of 47 kDa protein extracted from aged garlic (AGE) was evaluated on mouse dendritic cell (DC) maturation in vitro. Mate...

UNLABELLED BioBran, enzymatically modified arabinoxylan from rice bran was tested for its possible effects on in vitro maturation of human dendritic cells (DC). Immature DC (iDC) derived from plastic-adhered, IL-4 and GM-CSF treated peripheral monocytes (Mo) were further cultured with cytokine maturation mix 1 (CMM1; TNF-alpha, IL-1beta and IL-6) or CMM2 (LPS and IFN-gamma) to induce their matu...

1999;155:841–51. 24. Beyer M, Bartz H, Horner K, et al. Sustained increases in numbers of pulmonary dendritic cells after respiratory syncytial virus infection. J Allergy Clin Immunol 2004;113:127–33. 25. Brimnes MK, Bonifaz L, Steinman RM, et al. Influenza virus-induced dendritic cell maturation is associated with the induction of strong T cell immunity to a coadministered, normally nonimmunog...

variety of positive as well as negative regulatory signals are provided by antigen presenting cell in particular by dendritic cells. in this research, we studied the capacity of dendritic cells to expand antigen-specific t regulatory cells.we also investigated the role of tgf-beta in induction inhibitory functions of dendritic cells in mixed leukocyte reactions. dendritic cells were generated f...

INTRODUCTION Ankylosing spondylitis (AS) is a severe, chronic inflammatory arthritis, with a strong association to the human major histocompatibilty complex (MHC) class I allele human leucocyte antigen (HLA) B27. Disulfide-linked HLA-B27 heavy-chain homodimers have been implicated as novel structures involved in the aetiology of AS. We have studied the formation of HLA-B27 heavy-chain homodimer...

Glia maturation factor, a 17-kDa protein made up of 142 amino acid residues, mainly includes glia maturation factor-β (GMFB) and glia maturation factor-γ (GMFG). GMFB plays an important role in the growth and differentiation in neuron as well as glia. GMFG, a novel member of the glia maturation factor gene family, locates in the blood and immune system retaining proliferative and differentiated...