In this study, buffalo spermatozoa were in-vitro capacitated and acrosome reacted using modified Tyrode’s bicarbonatebuffered medium (sp-TALP media). Lipids were extracted from freshly ejaculated, in-vitro capacitated and acrosome reacted buffalo spermatozoa. The total lipid content of freshly ejaculated spermatozoa (171.5±2.90 μg/108 cells), capacitated spermatozoa (128.2±3.29 μg/108 cells) an...

This study investigated the development of rat oocytes in vitro and in vivo following intracytoplasmic injection of heads from spermatozoa heat-dried at 50°C for 8 h and stored at 4°C in different gas phases. Sperm membrane and chromosome are damaged by the process of heat-drying. Oocyte activation and cleavage of oocytes were worse in oocytes injected with spermatozoa heat-dried and stored for...

Utilization of cauda epididymis spermatozoa has been carried out because these can be used in artificial insemination or vitro fertilization. The purpose this study was to obtain information on the quality which produced from Bali cattle at different ages. samples came 31 slaughterhouses. Information and identification age based structure incisors. Observations were made variables motility, con...

This experiment was designed to study the effect of insulin like growth factor-I (IGF-I) on cryopreservation of Hariana bull semen. For this purpose, semen ejaculates were collected from four Hariana bulls using artificial vagina at biweekly interval. The semen samples which possess more than 70% initial progressive motility and above 500 million/mL spermatozoa concentration was subsequently su...

In the present study, the impact of non-functional spermatozoa on the cryopreservation success of functional boar spermatozoa was evaluated. Fifteen sperm-rich ejaculate fractions collected from five fertile boars were frozen with different proportions of induced non-functional sperm (0--native semen sample-, 25, 50 and 75% non-functional spermatozoa). After thawing, the recovery of motile and ...

This study examined whether washing and preincubation of boar spermatozoa was necessary to achieve high in vitro penetration rates of pig oocytes. In experiment 1, diluted, sperm-rich fractions, stored for 24 h at 16 degrees C (stored diluted sperm) were used. After centrifugation once at 50 g for 3 min, the supernatant was concentrated at 1200 g for 3 min. The pellets were washed (1200 g for 3...

OBJECTIVE Sperm morphology plays an important role in infertility, especially in cases of defects in the heads of spermatozoa. Tapered-head or elongated-head spermatozoa are examples of morphological abnormalities. The aim of this study was to compare the semen parameters, levels of protamine deficiency, and frequency of apoptosis between patients with normozoospermia and those with teratozoosp...

The motility characteristics and the behaviour of caput spermatozoa were investigated. After dilution in B2 medium the majority of spermatozoa presented head-to-head agglutination and a twisted flagellum. Only a small population of these spermatozoa developed an anarchic motility pattern. The addition of crude bovine epididymal fluid forward motility protein (FMP) to the incubation medium suppr...

The effect of iron on the attachment of gonococci to human spermatozoa was investigated using the three iron salts, ferric chloride, ferric nitrate, and ammonium ferric citrate (AFC). Ferric chloride and ferric nitrate were found to be unsuitable for such studies because they were insoluble at physiological pH values, produced a marked decrease in the pH of unbuffered medium (Ringer's solution)...

BACKGROUND The adverse effects on offspring of diabetic and/or obese mothers can be passed to the next generation. However, the mechanisms behind this are still unclear. Epigenetics may play a key role during this process. METHODS To confirm the hypothesis, we investigated the DNA methylation of several imprinted genes in spermatozoa of offspring from diabetic and/or obese mothers utilizing s...