The specific silver staining of nucleolar region organizers was applied to Daudi lymphoma control and interferon alpha-treated cells. Isolated nuclei from control and treated samples were used for the kinetic analysis of in vitro RNA and DNA synthesis. Results have shown that interferon treatment induces a reduction of the transcriptional and replicative activities within 90 min. Concomitant to...

To clarify the role of the multiple lineage leukemia gene-leukemia translocation gene of chromosome 19 (MLL-LTG19) protein in leukemogenesis, we synthesized antisense Oligodeoxyribonucleotide (ODN) against the fused region of the MLL-LTG19 chimi-rie transcript and treated KOCL33 cells carrying the t(ll;19) translocation with antisense ODN. The antisense ODN inhibited cell growth and induced apo...

The effects of B cell stimulatory factor 1 (BSF-1) on the generation of human CTL and lymphokine-activated killer (LAK) cells in vitro were investigated. Both L-2 and BSF-1 were potent helper factors for the generation of antigen-specific CTL in MLC; detection of optimal BSF-1-induced CTL activity in this system occurred when BSF-1 was added to cultures after an initial period of activation dur...

Abstract Human γδ T cells represent a small fraction of in peripheral blood (1–10%). They constitute unique subset lymphocytes that recognize non-peptide antigens through MHC-independent presentation. Major cell subsets, Vδ1 and Vδ2 possess distinct tissue localization, antigen recognition, effector responses. We hypothesized differences at the gene, phenotypic, functional level distinguish the...

To expand the spectrum of recognition of effector lymphocytes and to redirect them towards predefined targets, we have altered the specificity of human tumor-infiltrating lymphocytes (TIL) through stable modification with chimeric receptor genes consisting of single-chain antibody variable regions linked to the gamma subunit common to the immunoglobulin (Ig)G and IgE Fc receptors. Using either ...

Cytotoxic cells are produced in an autologous mixed leukocyte reaction (AMLR). At 1 wk in culture the AMLR killers are mainly IgG Fc- cells and can kill autologous lymphoblastoid cell lines and Raji and Daudi targets that are usually resistant to natural killer cell (NK) lysis. To define the phenotype of these cells, we have used complement (C')-mediated lysis with monoclonal antibodies (MAb). ...

Pavel Borodin, Jorge Brenes, Elias Daudi, Noor Efendi, Simon Flower, Muhammad Hidayat, Muhammad Husni, Manuel Kampine, Oleg Kusonski, Artur Langa, Iván Monge, Antonio Mucussete, Armindo Nhatsave, I Kadek Oca Santika, Jean Rasson, John Riddick, Didik Suharyadi, Christopher Turbitt, and Mahmud Yusuf Arti observatory, Institute of Geophysics, Urals Branch of Russian Academy of Sciences Instituto ...

The aim of the present study was to investigate the effects of 5-aminosalicyclic acid (5-ASA) on the cell injury mediated by activated neutrophils. We used a system constituted of neutrophils, triggered with phorbol myristate acetate, and 51Cr-labelled Daudi cells as targets. The results show that 5-ASA is capable of efficiently preventing neutrophil-mediated lysis. 5-ASA was up to 10-fold more...

An in vitro transcription system has been established with a whole-cell extract from the human Burkitt's lymphoma, Daudi, cell line. The B cell extract has been compared with a HeLa cell extract in an effort to study lymphocyte-specific regulatory factors of kappa light chain gene transcription. Both extracts were capable of transcribing Vk genes and other RNA polymerase II dependent genes. Alp...