The induction of haploid cell development into normal plants enables the production doubled lines, which are homozygous and can be used in breeding programs as an alternative to conventionally derived inbred lines. In this paper, we present historical background current status attempts carrot (Daucus carota L.). Economically, is one most important vegetables. It outcrossing diploid (2n = 2x 18)...

Haploid technologies are used to create homozygous lines for accelerated breeding. We aimed optimize the technology using isolated microspore culture in vitro obtain doubled haploids of carrot (Daucus carota L.).
 studied two varieties with different responsiveness embryogenesis, Altajskaya lakomka and Breeding line 17. Carrot microspores were from buds cultivated liquid nutrient media sup...

For restoration purposes, nature conservation generally enforces the use of local seed material based on the "local-is-best" (LIB) approach. However, in some cases recommendations to refrain from this approach have been made. Here we test if a common widespread species with no obvious signs of local adaptation may be a candidate species for abandoning LIB during restoration. Using 10 microsatel...

Arbuscular mycorrhizas (AM) are widespread, ancient endosymbiotic associations that contribute significantly to soil nutrient uptake in plants. We have previously shown that initial fungal penetration of the host root is mediated via a specialized cytoplasmic assembly called the prepenetration apparatus (PPA), which directs AM hyphae through the epidermis (Genre et al., 2005). In vivo confocal ...

Many crop species are able to hybridize with related weedy or wild relatives, which could lead to transfer of cultivar genes, and among them transgenes, into wild populations. It is not clear, however, whether the hybrids and their descendants are able to survive and reproduce in natural habitats, as inherited cultivar traits may be maladaptive under such conditions. To test this, we produced h...

Polyclonal antibodies raised against rat vesicle associated membrane protein-2 (VAMP-2) recognized, in carrot (Daucus carota) microsomes, two major polypeptides of 18 and 30 kD, respectively. A biochemical separation of intracellular membranes by a sucrose density gradient co-localized the two polypeptides as resident in light, dense microsomes, corresponding to the endoplasmic reticulum-enrich...

When following the membrane potential of Elodea densa leaf cells during a dark-light regime and analysing the different phases of the cycle, the pattern under boron deficiency resembled the one reported to occur after 3-(3,4-dichlorophenyl)-1,1-dimethylurea application. The potential in the dark slowly decreased when transferring Elodea densa leaflets and Helianthus annuus roots to a B-free med...

Gamma irradiation is a form of pure energy which is currently used most widely for food and waste irradiation. To evaluate the potential of gamma irradiated sludge for its suitability as a soil amendment in agriculture, field experiments were carried out in a root crop, carrot (Daucus carota). Treatments consisting of three sources of manure (Farmyard Manure (FYM), gamma-irradiated sewage sludg...

A procedure is described for the isolation of native DNA from protoplasts of ammi (Ammi visnaga) and carrot (Daucus carota) cells. Protoplasts were produced from 40 grams of fresh cells by enzyme hydrolysis and lysed with sodium dodecyl sulfate. The DNA was purified by treatment with pronase and ribonuclease. Final isolation was achieved by sucrose density gradient centrifugation.The melting te...

When disks of carrot (Daucus carota) phloem parenchyma are incubated for 6 days there is a 10-fold increase in cell wall hydroxyproline due to the synthesis and secretion of hydroxyproline-containing macromolecules. The synthesis of these molecules and their secretion are demonstrated by measuring the kinetics of incorporation and of chase of (14)C-proline and hydroxyproline in different fracti...