Cytochrome c oxidase (EC 1.9.3.1) is the terminal enzyme of the mitochondria respiratory chain catalysing electron transfer from cytochrome c to molecular oxygen [ 1,2]. The molecular mechanism of this process is still not understood. At present, little is known about such important structural features as the position of the prosthetic groups or the location and characteristics of the cytochrom...

The terminal enzyme of the mitochondrial respiratory chain, cytochrome oxidase, transfers electrons to molecular oxygen, generating water. Within the inner mitochondrial membrane, cytochrome oxidase assembles into supercomplexes, together with other respiratory chain complexes, forming so-called respirasomes. Little is known about how these higher oligomeric structures are attained. Here we rep...

Using near-infrared spectroscopy, we developed a new approach for measuring the redox state of cytochrome oxidase in the brain under normal blood-circulation conditions. Our algorithm does not require the absorption coefficient of cytochrome oxidase, which differs from study to study. We employed this method for evaluation of effects of changes in oxygen delivery on cerebral oxygenation in rats...

The use of near-infrared spectroscopy to measure noninvasively changes in the redox state of cerebral cytochrome oxidase in vivo is controversial. We therefore tested these measurements using a multiwavelength detector in the neonatal pig brain. Exchange transfusion with perfluorocarbons revealed that the spectrum of cytochrome oxidase in the near-infrared was identical in the neonatal pig, the...

Many cyanobacteria species can use both plastocyanin and cytochrome c6 as lumenal electron carriers to shuttle electrons from the b6f either photosystem I or respiratory c oxidase. In Synechocystis sp. PCC6803 placed in darkness, about 60% of active PSI centres are bound a reduced donor which is responsible for fast re-reduction P700 vivo after single charge separation. Here, we show that bind ...

When preparations of cytochrome c from mammalian species are chromatographed on columns of weak cation exchange agents, more than one chromatographic species can be obtained (1, 2). Only one of these fractions, that elutcd from the polycarboxylic resin Amberlite IRC-50 (Rohm and Hnas Company) at sodium or ammonium ion concentrations of 0.22 to 0.25 equivalent per liter (2, 3), can be considered...

Comparative assessment of the mitochondrial cytochrome oxidase I (mtCOI) gene sequence uncovers Bemisia tabaci mitotypes. The current study aims to define B. mitotypes found in various agro ecological zones across India. Phylogenetic analysis 13 samples from agroclimatic regions revealed presence four mitotypes: Asia 1, II 5 and 7. Among, 1 was be abundant widespread distribution thirteen locat...

Phagocytic leukocytes contain an activatable NADPH:O2 oxidoreductase. Components of this enzyme system include cytochrome b558, and three soluble oxidase components (SOC I, SOC II, and SOC III) found in the cytosol of resting cells. Previously, we found that SOC II copurifies with, and is probably identical to, a 47-kDa substrate of protein kinase C. In the present study we investigated the cha...

S100A8 and S100A9 are two calcium binding Myeloid Related Proteins, and important mediators of inflammatory diseases. They were recently introduced as partners for phagocyte NADPH oxidase regulation. However, the precise mechanism of their interaction remains elusive. We had for aim (i) to evaluate the impact of S100 proteins on NADPH oxidase activity; (ii) to characterize molecular interaction...