The advent of phototransformable fluorescent proteins has led to significant advances in optical imaging, including the unambiguous tracking of cells over large spatiotemporal scales. However, these proteins typically require activating light in the UV-blue spectrum, which limits their in vivo applicability due to poor light penetration and associated phototoxicity on cells and tissue. We repor...

The absorption and fluorescence spectra of five typical cyanine dyes adsorbed on the surface of TiO colloids have been 2 studied. The absorption spectra show that there are two kinds of isomer produced by intramolecular torsional motion of these dyes in their ground states. The fluorescence quantum yields of these dyes may be enhanced or reduced in the presence of TiO colloids. This behavior ca...

The development of fluorescent probes for superoxide and the hydroxyl radical is a central problem in the field of chemical biology. Superoxide and the hydroxyl radical play a significant role in a variety of inflammatory diseases, and probes that can detect these reactive oxygen species (ROS) have tremendous potential as medical diagnostics and research tools. Fluorescent sensors for superoxid...

Cyanine dyes are broadly used for fluorescence imaging and other photonic applications. 3,3'-Diethylthiacyanine (THIA) is a cyanine dye composed of two identical aromatic heterocyclic moieties linked with a single methine, -CH 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 000000000000000000000000000000...

In the past few years, applications built around two-photon absorption (2PA) have emerged, which require new materials to be designed and characterized in order to discover new applications and to advance the existing ones. This chapter describes the nonlinear optical processes and characterization techniques along with design strategies and structure–property relations of cyanine and cyanineli...

The excited-state dynamics of the DNA bisintercalator YOYO-1 and of two derivatives has been investigated using ultrafast fluorescence up-conversion and time-correlated single photon counting. The free dyes in water exist in two forms: nonaggregated dyes and intramolecular H-type aggregates, the latter form being only very weakly fluorescent because of excitonic interaction. The excited-state d...

X Ž . The photophysics and isomerization dynamics in the excited state of a cyanine dye, 3,3 -di 3-sulfopropyl -thiacyanine triethylaminium salt was investigated. Using the femotosecond time-resolved fluorescence up-conversion technique, wavelength-dependent fluorescence dynamics was observed and the theoretical prediction of the dual fluorescence behavior was vindicated. The results were expla...