The aqueous phase in phenol extracts of HeLa cells contain deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and polyglucose (CHO), (Segovia et al. , 1965). -RNA reacts with the CHO and they coprecipitate in 20% ethanol-saline solutions (Graves et al., 1967). -Native DNA precipitates when the ethanol concentration is increased to 50%. Although native DNA and CHO have equal buoyant densities ...

BACKGROUND/OBJECTIVES The main objective of this study was to evaluate the effects of a high cholesterol (HC) dietary challenge on cholesterol tissue accumulation, inflammation, adipocyte differentiation, and macrophage infiltration in guinea pigs. A second objective was to assess whether macronutrient manipulation would reverse these metabolic alterations. MATERIALS/METHODS Male Hartley guin...

BACKGROUND Methylotrophic (methanol-utilizing) bacteria offer great potential as cell factories in the production of numerous products from biomass-derived methanol. Bio-methanol is essentially a non-food substrate, an advantage over sugar-utilizing cell factories. Low-value products as well as fine chemicals and advanced materials are envisageable from methanol. For example, several methylotro...

Amino acids interact with glucose metabolism both as carbon substrates and by recycling glucose carbon via alanine and glutamine; however, the effect of protein intake on glucose homeostasis during weight loss remains unknown. This study tests the hypothesis that a moderate increase in dietary protein with a corresponding reduction of carbohydrates (CHO) stabilizes fasting and postprandial bloo...

The cho-1 gene in Caenorhabditis elegans encodes a high-affinity plasma-membrane choline transporter believed to be rate limiting for acetylcholine (ACh) synthesis in cholinergic nerve terminals. We found that CHO-1 is expressed in most, but not all cholinergic neurons in C. elegans. cho-1 null mutants are viable and exhibit mild deficits in cholinergic behavior; they are slightly resistant to ...

The human foamy virus (HFV) genome possesses three open reading frames (bel 1, 2, and 3) located between env and the 3' long terminal repeat. By analogy to other human retroviruses this region was selected as the most likely candidate to encode the viral transactivator. Results presented here confirmed this and showed further that a deletion introduced only into the bel 1 open reading frame of ...

RATIONALE The participation of endothelial cells (EC) in many physiological and pathological processes is widely modeled using human EC cultures, but genetic manipulation of these untransformed cells has been technically challenging. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 nuclease (Cas9) technology offers a promising new approach. However,...

The great advantage of the tetracycline-inducible system lies in its ability to address a large variety of biological questions in a time-dependent and tissue-specific manner. This study describes a transgenic mouse line, rTA(LAP)-1, which produces the reverse tetracycline transactivator under control of the liver activator protein (LAP) promoter. Two reporter lines with luciferase and LacZ rep...

Hyunjeong Cho, Yongjin Yi, Eunjeong Kang, Seokwoo Park, Eun Jin Cho, Sung Tae Cho, Rho Won Chun, Kyu Eun Lee, Kook-Hwan Oh Department of Internal Medicine, Seoul National University College of Medicine, Seoul; Department of Internal Medicine, Hongseong Medical Center, Hongseong; Dr. Chun & Cho`s Medical Clinic & Dialysis Center; Department of Surgery, Seoul National University College of Medici...