نتایج جستجو برای: culture method
تعداد نتایج: 1875618 فیلتر نتایج به سال:
Astrocytes are an abundant cell type in the mammalian brain, yet much remains to be learned about their molecular and functional characteristics. In vitro astrocyte cell culture systems can be used to study the biological functions of these glial cells in detail. This video protocol shows how to obtain pure astrocytes by isolation and culture of mixed cortical cells of mouse pups. The method is...
Background: Infectious diseases are problematic in all around the world especially in the developing countries and early diagnosis of infections and one etiologic agents has a major role in the treatment of one patients. There are some culturing methods consist of conventional, semiautomatic and automatic. One of automatic methods is BACTEC system worked by fluorescent technology and Co2 produc...
We compared the infectivity and growth rates of 12 strains of Orientia tsutsugamushi in a cell culture system. ECV304 cells were infected with O. tsutsugamushi strains for 4 hr, and the culture was maintained for 72 hr. Immunofluorescence (IF) staining was performed at 4, 24, 48, and 72 hr after inoculation (hpi), and IF-positive foci were enumerated. The AFSC-4 strain, which is known to be ins...
Sheeppox virus from an outbreak of sheeppox that occurred in Srinagar (Jammu and Kashmir, India) in 2000 was isolated by inoculation of susceptible sheep and further re-isolated in cell culture. The field virus, adapted to grow in lamb testes culture, was evaluated for its potential use as challenge virus in potency testing of sheeppox vaccine currently in use. The virus (passage 6) produced se...
Introduction: Safety in construction needs not only operating the executive laws and instructions but also the safety culture of all the workers in workshops. Safety culture is a factor by which all the workers are committed to having a positive share in the safety of their coworkers and themselves. Therefore, the aim of this study is identification of safety culture factors and ranking occupat...
Purpose: The aim of the present research is to identify the organizational culture in the organization of documents and the National Library of the Islamic Republic of Iran by using Robin’s model. Method: The research method is an analytical survey method. The population consists of 609 staff members of the organization of documents and national library of Iran who have at least an associate d...
In the present investigation Jatropha and pea plant leafs was used, to germinated the plant by artificial condition through plant tissue culture method. We have taken three methods Method A, B & C to germinate callus, seed, bud and stem. 30 Sample were taken to analyze. In Method A 60% samples were contaminated (fungus and bacteria) and 20% was shown growth. In Method B 66% was contaminated by ...
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