Nhumirim virus (NHUV) is an insect-specific virus that phylogenetically affiliates with dual-host mosquito-borne flaviviruses. Previous in vitro co-infection experiments demonstrated prior or concurrent infection of Aedes albopictus C6/36 mosquito cells with NHUV resulted in a 10,000-fold reduction in viral production of West Nile virus (WNV). This interference between WNV and NHUV was observed...

The Culex pipiens complex consists of vector mosquitoes that transmit important human pathogens. In this study we established a simplified method to distinguish three members of the Cx. pipiens complex, Cx. p. pallens Coquillet, Cx. p. form molestus Forskal, and Cx. quinquefasciatus Say, collected in Japan. Sequence analysis of the Drosophila Ace-orthologous acetylcholinesterase (Ace) gene (668...

In the last 5 years, there has been only one reported human case of West Nile virus (WNV) disease in northern Mexico. To determine if the virus was still circulating in this region, equine and entomological surveillance for WNV was conducted in the state of Nuevo Leon in northern Mexico in 2006 and 2007. A total of 203 horses were serologically assayed for antibodies to WNV using an epitope-blo...

G-protein-coupled receptors regulate signal transduction pathways and play diverse and pivotal roles in the physiology of insects, however, the precise function of GPCRs in insecticide resistance remains unclear. Using quantitative RT-PCR and functional genomic methods, we, for the first time, explored the function of GPCRs and GPCR-related genes in insecticide resistance of mosquitoes, Culex q...

A study was conducted to determine the susceptibility of local strains of Culex quinquefasciatus and Aedes aegypti to infection with the strain of Wuchereria bancrofti that occurs in Maceió. State of Alagoas, Brazil. Cx. quinquefasciatus blood fed simultaneously on the same microfilariae carrier ingested more blood and 2-3x more microfilariae than Ae. aegypti. Survival rates of both species of ...

Mosquito have the ability of carrying and transmitting human and animal diseases across the countries causing hundreds of millions of clinical cases and millions of death annually[1,2]. Among several species of mosquitoes Culex quinquefasciatus (Diptera: Culicidae) (Cx. quinquefasciatus) is main periodic vector of filarial parasite Wuchereria bancrofti, accredited for human lymphatic filariasis...

Studies on the vector competence and their role in the transmission of malaria and filariasis in Makurdi, Nigeria were carried out over a 6 month period in four localities. A total of 475 adult female mosquitoes were morphologically identified and dissected using standard procedures to determine the parity rates, insemination rates, age structure, oocyst, sporozoite and microfilarial worms. 156...

The efficacy of sustained release Altosid sand granules to control adult Aedes taeniorhynchus and Culex quinquefasciatus emergence was investigated. Sand granules applied at a 7-day preflood application rate of 5.6 kg/ha controlled 99% of the Ae. taeniorhynchus emergence in saltwater plots for 44 days posttreatment and 35% of Cx. quinquefasciatus in freshwater plots. A 5.6 kg/ha rate controlled...

To classify mosquito species based on common features of their habitats, samples were obtained fortnightly between June 2001-October 2003 in the subtropical province of Chaco, Argentina. Data on the type of larval habitat, nature of the habitat (artificial or natural), size, depth, location related to sunlight, distance to the neighbouring houses, type of substrate, organic material, vegetation...

Mosquitoes are vectors of several human pathogens, and great attention has recently been placed on insecticides from plant-derived products, in search for mosquito control agents. This study, thus, investigated the potency of Boswellia dalzielii methanol leaf extract and its four fractions as mosquito ovicide, larvicide, and pupicide against Anopheles gambiae and Culex quinquefasciatus. The pla...