Matrix metalloproteinase 2 (MMP-2) in metastatic cancer tissue, which is associated with a poor prognosis, is a potential target for tumor imaging in vivo. Here, we describe a metastatic cancer cell-targeted protein nanocage. An MMP-2-binding peptide, termed CTT peptide (CTTHWGFTLC), was conjugated to the surface of a naturally occurring heat shock protein nanocage by genetic modification. The ...

The formation of intramolecular triplex DNA can be regulated by Ag(+) and Cys (cysteine), which switch off/on the fluorescence of the oligonucleotides, 5'-TAMRA-TTC TCT TCC TCT TCC TTC TGA CGA CAG TTG ACT CTT CCT TCT CCT TCT CTT-BHQ-2-3' (Oligo 1) and 3'-GAA GGA AGA GGA AGA GAA-5' (Oligo 2). Based on this principle, sensors for Ag(+) and Cys are developed. The sensor for Ag(+) has a linear rang...

5' AAGCTTCCAC TACCTTGCCA CCCGCAATAA GAACGATTAC TTCTCCCTCG CCTT 54 Kec Pro tie Thr Asn 141 CTACCA CCTAAAGATC TCCCTCTTAT TTTTAGOTTG AACTCGTATA AACCAAAATT AATTACACCA GATAAA ATG CCA ATT ACA AAT -35 -10 BBS fut Lys ThT Hec Leu Il« Thr Tyr Ala Aap Ser Leu Gly Lye Asa Leu Lys Glu Leu Asn Glu A«n H e Glu Agn Tyr AAA ACA ATG TTC ATT ACT TAC GCA CAC ACT TTC OGT AAA AAT TTG AAA CAA TTG AAT GAA AAT ATT GAG...

Subjective health measurements are increasingly used in clinical research, particularly for patient groups comparisons. Two main types of analytical strategies can be used for such data: so-called classical test theory (CTT), relying on observed scores and models coming from Item Response Theory (IRT) relying on a response model relating the items responses to a latent parameter, often called l...

Despite the broadly conserved role of microtubules in chromosome segregation, we have a limited understanding of how molecular features of tubulin proteins contribute to the underlying mechanisms. Here we investigate the negatively charged carboxy-terminal tail domains (CTTs) of α- and β-tubulins, using a series of mutants that alter or ablate CTTs in budding yeast. We find that ablating β-CTT ...

OBJECTIVE Despite a growing call to use patient-reported outcomes in clinical research, few are available for measuring upper limb function post-stroke. We examined the Disabilities of the Arm, Shoulder and Hand (DASH) to evaluate its measurement performance in acute stroke. In doing so, we compared results from traditional and modern psychometric methods. METHODS 172 people with acute stroke...

BACKGROUND/AIM Functional constipation (FC) and constipation-predominant IBS (C-IBS) are two main subtypes of constipation. Using radio-opaque markers is an easy and cost effective method to measure colonic transit time (CTT). We designed this study to compare the CTT between these two groups of constipated patients. METHODS Patients with chronic constipation of no organic etiology were class...

The purpose of this paper was to evaluate the psychometric properties of a stage-specific self-efficacy scale for physical activity with classical test theory (CTT), confirmatory factor analysis (CFA) and item response modeling (IRM). Women who enrolled in the Women On The Move study completed a 20-item stage-specific self-efficacy scale developed for this study [n=226, 51.1% African-American a...

The effects of different language backgrounds on trail making tasks were examined in this study. The measures adopted were the Color Trails Test (CTT) and the Trail Making Test (TMT), the former being the culturally fair analogue of the latter. Eighty-four right-handed participants belonging to the Chinese-English Bilingual (CEB) and English Monolingual (EM) groups participated in this study. T...

The traditional method for construct development in information systems (IS) is based on classical test theory (CTT). While the Rasch approach is more theoretically sound than CTT, it has not been widely adopted in the IS literature. This is probably because CTT and Rash often produce similar results but Rash it is more difficult to perform. However, test equating, an important aspect of Rasch ...