Src homology 2 domain-containing protein tyrosine phosphatase [SHP] substrate 1 (SHPS-1), a receptor-type transmembrane glycoprotein whose cytoplasmic region binds and activates the protein tyrosine phosphatases SHP-1 and SHP-2, and thereby modulates multiple cellular functions. Its extracellular region regulates intercellular communication in the neural and immune systems through its associati...

C218 located at the interface between A-band and M-line. It has been shown by Centner et al. [2] that MURF-1, a member of the RING finger proteins, binds to the two Ig-domains A168 and A169 in proximity to the kinase. Thus, its binding might be involved in the regulation of titin kinase. The structure of this tandem Ig domain has been solved. Ig domains, also in titin, are involved in many prot...

Heteropolytungstates play a dual role in ribosomal crystallography. Beside generating phases, one of them, was found to be extremely useful in inducing post crystallization rearrangements. These led to a significant increase in the internal order of crystals of the small ribosomal subunits from Thermus thermophilus, manifested in a dramatic extension of the resolution of their diffraction patte...

Everything in Nature, macroscopic or microscopic, inorganic, organic or biological, has its specific properties. Most properties of matter depend on the structures, and many techniques have been developed over the centuries for structure analysis. The greatest of them all, structure analysis of single crystals by X-ray diffraction, X-ray crystallography, was founded in 1912 and is still today t...

The CCP4 Study Weekend 2006 covered a wide range of topics associated with structural studies of complexes between proteins, proteins and nucleic acids and proteins and small molecules. The programme was composed of lectures from different areas of structural biology including: preparation and characterization of complexes for structural analysis, co-crystallization and soaking techniques, mole...

A new chip-based crystal-mounting approach for rapid room-temperature data collection from numerous crystals is described. This work was motivated by the recent development of X-ray free-electron lasers. These novel sources deliver very intense femtosecond X-ray pulses that promise to yield high-resolution diffraction data of nanocrystals before their destruction by radiation damage. Thus, the ...

Carboxypeptidase Y (CPY) inhibitor I from the yeast, consisting of 204 amino acid residues, belongs to the phosphatidylethanolaminebinding protein (PEBP) family. The 2.7 Å crystal structure of the ICPY complex has been solved by molecular replacement [1, 2]. The structure of I consists of a major -type domain and an Nterminal helical segment. I has two CPY-binding sites: the N-terminal inhibito...