The goal of this comparative study was to investigate biofilm forming microorganisms living in washing machines (WMs). Biofilms were sampled from 11 washing machines from four countries and three continents. Among the 94 isolated strains, 30% were potential human pathogens. Representative strains were selected and biofilm formation was evaluated with the crystal violet (CV) assay. The majority ...

In previous work, Vibrio proteolyticus 5S rRNA was shown to stabilize 13-50 nucleotide "guest" RNA sequences for expression in Escherichia coli. The expressed chimeric RNAs accumulated to high levels in E. coli without being incorporated into ribosomes and without obvious effects on the host cells. In this work, we inserted sequences encoding known aptamers recognizing a protein and an organic ...

The results of a study and application of leucocrystal violet for the determination of antimony in parts per million levels is described here. The proposed method is based on the reaction of antimony(III) with acidified potassium iodate to liberate iodine. The liberated iodine selectively oxidizes leucocrystal violet to crystal violet dye. The formed dye shows maximum absorbance at 590 nm. The ...

Background: Candida albicans is the most common bacteria related to root canal treatment failure. Avocado seeds (Persea americana) contain terpenoids, alkaloids, flavonoids, steroids, saponins and tannins that have antibacterial activity. Purpose: To determine effect of avocado seed ethanol extract on C.albicans biofilm. Methods: The ATCC 10231 biofilm was evaluated by crystal violet assay. wer...

results the minimum inhibitory concentrations (mics) of ginger extract for evaluated strains were 40, 40, 20, 20, 20, 20, 10, and 5 mg/ml for pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, klebsiella pneumoniae, bacillus cereus, acinetobacter baumannii, c. albicans, and c. krusei, respectively. ginger extract successfully inhibited biofilm formation by a. baumannii, b. cereus,...

conclusions: it can be concluded that most of a. baumannii isolates can form biofilm in microtiter plate and tube. the results also verified that most of these isolates were resistant to ciprofloxacin and imipenem. results: results showed that in tube method under shaking, 22% of clinical isolates were strong biofilm producers while 23% of them were not able to form biofilms. in this experiment...

High mobility group box 1 (HMGB1) was originally defined as a nuclear protein. However, later studies showed that HMGB1 was released from damaged cells into the extracellular milieu and functioned as a danger signaling molecule. HMGB1 has also been shown to exert proliferative and chemoattractant effects on many cell types. In this study, we investigated the in vitro effect of human recombinant...

The purpose of this study is to evaluate four rapid colourimetric methods, including the resazurin microtitre assay (REMA), malachite green decolourisation assay (MGDA), microplate nitrate reductase assay (MNRA) and crystal violet decolourisation assay (CVDA), for the rapid detection of multidrug-resistant (MDR) tuberculosis. Fifty Mycobacterium tuberculosis isolates were used in this study. Ei...

This study examines substances from plant leaves of Azadirachta indica and Melia azedarach extracted with methanol (80%) then successively fractionated increasing gradient polarity solvents. The extract the two was selected to be incorporated nanocomposites because their high antimicrobial activities. HPLC analysis performed for determination total phenolics flavonoids. LC–ESI–MS positive ion a...

Lactobacillus rhamnosus are Gram-positive and lactic acid-producing bacteria. Meanwhile, Candida albicans tropicalis opportunistic fungi that cause oral candidiasis. This study aimed to determine the effect of LGG on biofilm formation C. with hypothesis inhibits yeasts. ATCC MYA-4901 13803 were standardised 1x106 cells form a mono-species biofilm. L. GG (LGG) was 1x107 cells, equivalent absorba...