Early menopause and infertility often occur in female cancer patients after chemotherapy (CTx). For these patients, oocyte/embryo cryopreservation or ovarian tissue cryopreservation is the current modality for fertility preservation. However, the above methods are limited in the long-term protection of ovarian function, especially for fertility preservation (very few females with cancer have ac...

Cryopreservation of human gametes and embryos has become an essential part of assisted reproduction. It is now possible to cryopreserve gametes and embryos at their different stages of development. It allows patients undergoing chemotherapy or radiotherapy to preserve their fertility, and helps to attain all benefits from the costly ovarian superovulation therapies prior to assisted reproductiv...

Transplantation of cryopreserved ovarian tissue is a technology that holds promise for preserving reproductive potential for the future. It may be apropos for cancer survivors who will undergo treatment with sterility-inducing chemotherapy or radiation. Although there is some evidence suggesting cellular and molecular injury with the freezing and thawing process, there are examples in both anim...

objective: this study aimed to evaluate the incidence of apoptosis in vitrified and non-vitrified human ovarian tissue by the use of morphological analysis and apoptosis assay techniques. methods: we obtained human ovarian tissue biopsies from 30 women who underwent elective caesarean sections. tissues were transported to the laboratory in pre-warmed, equilibrated leibovitz l-15 medium within 2...

The development of new method to cryopreserve human ovarian cortex tissues without damage is needed for the improvement of quality of life (QOL) of female cancer patients. Here we show novel cryopreservation method of human ovarian cortex tissues by using supercooling (S.C.) procedure. Our method will be helpful in order to preserve fertility of female cancer patients.

Background: Ovarian tissue cryopreservation is a feasible method to preserve female reproductive potential, especially in young patients with cancer or in women at risk of premature ovarian failure. Vitrification has recently emerged as a new trend for biological specimen preservation. On the other hand, gene expression that changes during vitrification can influence oocyte maturation and need ...

In 2010, there were an estimated 730,000 new cancer cases in females, resulting in approximately 270,000 deaths [ 1 ] . Although malignancy remains a critical health concern, signi fi cant medical advances in cancer detection and treatment have improved survival rates for patients. Gonadotoxic chemotherapy or radiation for malignant disease may result in altered ovarian function, affecting both...

As the chapters in this volume demonstrate, like other nascent medical technologies ovarian tissue cryopreservation (OTC) raises no earth-shatteringly new moral questions. Rather, it poses old moral questions in new ways, thus shedding light not only on our old answers but also on our old methods of reaching them. My task here is to point out the ways in which OTC forces us to embrace important...

Despite reasonable achievements in different animal species, the debate about many technical aspects of ovarian tissue banking is continuing. Human ovarian tissue banks are increasingly established around the world without a clear plan about how to make the best use of such tissue. One of the important challenges facing this growing technology is to determine the ideal method for the use of thi...

to the editor: Premenopausal women who undergo high-dose chemotherapy have a very high risk of ovarian failure. 1 Cryopreservation of ovarian tissue with subsequent autotransplantation has effectively preserved fertility in an animal model, 2 but its efficacy in humans has been uncertain. Eggs that were aspirated from cryopreserved ovarian tissue transplanted in heterotopic sites did not result...