Crosslinked organic and hybrid multilayer thin films based on polystyrene-grafted platinum nanoparticles and azidomethyl-functionalized polystyrenes are built-up by sequential spin-coating and UV crosslinking processes. This approach allows to easily tune thickness, composition and periodicity of each layer, as highlighted by TEM and neutron reflectivity experiments. Room temperature UV crossli...

Two novel cyclic quaternary amine crosslinking probes are synthesized for structural mass spectrometry of protein complexes in solution and for analysis of protein interactions in organellar and whole cell extracts. Each exhibits high aqueous solubility, excellent protein crosslinking efficiencies, low collision induced dissociation (CID) energy fragmentation efficiencies, high stoichiometries ...

We present a rapid and highly efficient method to form microstructure of poly(ethylene glycol) (PEG)-based acrylates by microwave-induced thermal crosslinking. PEG-based polymeric microstructures such as polymer microarrays and microwells were fabricated on 3-(trimethoxysilyl)propyl methacrylate (TMSPMA)-coated glass slides that were placed on top of a silicon wafer. In comparison to ultraviole...

Mucoadhesive chitosan microspheres of amlodipine besylate were prepared for nasal administration with the aim of avoiding the first pass effect. A series of batches of microspheres were prepared by simple emulsification crosslinking method to optimize parameters like external phase (mixture of heavy and light liquid paraffin in the ratio of 1:1), stirring rate (1200 rpm), dioctyl sodium sulfosu...

Crosslinking proteins maintain organelle structure and facilitate their function through the crosslinking of cytoskeletal elements. We recently found an interaction between the giant crosslinking protein dystonin-a2 and the microtubule-associated protein-1B (MAP1B), occurring in the centrosomal region of the cell. In addition, we showed that this interaction is necessary to maintain microtubule...

The structural requirements for signal transduction by class I major histocompatibility complex (MHC) molecules were examined. Native or mutant HLA-A2 or HLA-B27 constructs lacking most of their cytoplasmic domains were co-transfected with pSV2neo into Jurkat cells. Transfection of either native or mutant constructs resulted in a comparable expression of the gene products. Stimulation of transf...

For the first time, gliadin films with excellent strength and water stability have been developed without using any crosslinking agents. So far, it has not been possible to obtain water stable gliadin films even after crosslinking. In this research, a novel method of using aqueous urea and ethanol has been developed to obtain highly water stable gliadin films without using crosslinking chemical...

Maleic acid (MA) has been explored to replace formaldehyde-based dimethylol dihydroxy ethylene urea (DMDHEU) for cotton anti-crease finishing. However, the resilience of treated fabrics was not satisfactorily improved. In this study, acryloyl malic acid (AMA) was synthesized and applied on fabrics as a novel crosslinking agent. The results showed that both crease recovery angle and whiteness in...