Creatine Kinase (CK) is the enzyme responsible for catalysing the exchange of phosphates in the creatine/phosphocreatine shuttle. Skeletal muscle CK is made up of 90% muscle CK (CKMM) and 10% heart CK (CK-MB). In muscle damage (e.g. rhabdomyolysis), extreme exercise and statin-induced myositis, serum CK can be raised in excess of 10 times the upper limit of normal. However, the recent discovery...

IMPORTANCE Abnormalities in neural activity and cerebral bioenergetics have been observed in schizophrenia (SZ). Further defining energy metabolism anomalies would provide crucial information about molecular mechanisms underlying SZ and may be valuable for developing novel treatment strategies. OBJECTIVE To investigate cerebral bioenergetics in SZ via measurement of creatine kinase activity u...

An automated method has been devised for determining 5’-nucleotidase activity in serum. A modified creatine phosphokinase cartridge (Technicon Instrument Corp., Tarrytown, N. Y.) is used to prepare a reaction mixture containing, per liter: barbital buffer (pH 7.5), 16.1 mmol; adenosine-5’-phosphate, 5.4 mmol; Mg2, 20.2 mmol; phenyl phosphate, 7.8 mmol; adenosine deamlnase, 0.78 mg; and sample, ...

Pretreatment of adenosine diphosphate-G-actin solutions with apyrase results in a marked decrease in their rate of polymerization at 0” induced by KCl, creatine phosphate, and creatine kinase. In contrast, apyrase has no effect on the rate of polymerization of ATP-G-actin. The retardation can be reversed by the addition of a low concentration of ADP or of ATP. By comparing the rate of polymeriz...

The Na(+),Cl(-),creatine transporter CreaT (SLC6A8) mediates concentrative cellular uptake of creatine into a wide variety of cells. Previous observations disclosed that SLC6A8 transport activity is enhanced by mammalian target of rapamycin (mTOR) at least partially through the serum and glucocorticoid inducible kinase isoforms SGK1 and SGK3. As SLC6A8 does not contain a putative SGK consensus ...

The utilization of the energy of the oxidative processes of the Krebs tricarboxylic acid cycle for the synthesis of compounds with high energy phosphate bonds (adenosine triphosphate (ATP) or creatine phosphate) has been demonstrated in vitro with tissue extracts, slices, and homogenates (l-7). Furthermore, it might be expected that if oxidative reactions were accelerated in the intact rat an i...

Cardiac failure appears rapidly during severe hypoxia and precedes a substantial reduction in adenosine triphosphate content. Reduced adenosine triphosphate turnover, in the presence of nearly normal content, may be the metabolic basis for contractile failure during hypoxia. To measure both the myocardial content and the turnover rates of high-energy phosphate compounds during hypoxia, we perfo...

ATP-dependent calcium sequestration was previously localized in vesicles of mitotic apparatus isolated from sea urchins. We now demonstrate that the mitotic apparatus contains an ATP-regenerative system characterized as creatine kinase (EC 2.7.3.2). Mitotic apparatus isolated with vesicles intact converted ADP to ATP if phosphocreatine was present. Omission of ADP or phosphocreatine gave neglig...

Magnetic resonance spectroscopy (MRS) is used to determine the quantity of metabolites, such as creatine phosphate (PCr) and adenocine triphosphate (ATP), in the living body by collecting their nuclear magnetic resonance (NMR) spectra. In the field of MRS, the frequency spectrum of metabolites is usually obtained by applying the fast Fourier transform (FFT) (Cooley & Tukey, 1965) to the NMR sig...

New types of vinegar drinks are being processed and developed using various raw materials. In this study, a beverage containing mixture 0.25% red ginseng extract 5% Prunus mume fruit (RPV) was evaluated for its anti-fatigue effect on high-intensity exercised rats. RPV administration markedly enhanced running endurance significantly decreased fatigue-related serum biomarkers, such as inorganic p...