Creatine phosphokinase (CK) (ATP: creatine phosphotransferase, ЕС 2.7.3.2.) is found in a variety of cells with high and fluctuating energy requirements. It catalyses the reversible transfer of the high-energy-N-phosphoryl group from phosphocreatine to ADP. Creatine kinase connects sites of energy production with sites of energy consumption (Dolder et. al., 2001; Focant et al., 1970; Grossmann ...

We report the expression of the human muscle (CK-MM) and brain (CK-BB) creatine kinases in Escherichia coli. The proteins have been purified to apparent homogeneity and several of their physical and kinetic properties investigated. In the process, we have conclusively verified the correct DNA sequence of the genes encoding the respective isozymes, and determined the correct primary structure an...

Muscle is the major consumer of fuels and ATP in the body. Mitochondria and glycolytic complexes serve as the main energy production locations, while the highest energy demands are associated with the sarcoplasmic reticulum, myofibrillar compartments and plasma membrane. Creatine kinase (CK) is a dimeric protein, which is deeply involved in the production of high energy storage compounds. This ...

Previous studies have shown that creatine supplementation reduces muscle damage and inflammation following running but not following high-force, eccentric exercise. Although the mechanical strain placed on muscle fibers during high-force, eccentric exercise may be too overwhelming for creatine to exert any protective effect, creatine supplementation may protect skeletal muscle stressed by a res...

Creatine is a principle component of the creatine kinase (CK) phosphagen system common to all vertebrates. It is found in excitable cells, such as cardiomyocytes, where it plays an important role in the buffering and transport of chemical energy to ensure that supply meets the dynamic demands of the heart. Multiple components of the CK system, including intracellular creatine levels, are reduce...

Serum enzymes (aspartate transaminase, alanine transaminase, alkaline phosphatase (ALP), gamma-glutamyltransferase, and creatine kinase (CK] were measured in 296 young persons who admitted to recent inhalation of solvents, usually toluene based glues. In general, results fell within expected adult reference ranges except for ALP and CK. About 60% of subjects had CK activities above the upper re...

Gene variants, such as creatine kinase (CK) polymorphisms, have been suggested to explain the inter-individual CK response. However, this association is still unclear. Therefore, the purpose of this study was to analyze the association between the magnitudes of the CK response to exercise with the occurrence of muscle-CK-MM NcoI polymorphism in young healthy subjects. Blood CK activity was asse...

Patterns of creatine kinase (CK, EC 2.7.3.2) isoenzymes were studied in apparently healthy one- to 10-day-old neonates, by use of a sensitive fluorescent staining method with Sclavo CK-F/6001 reagent. Mean activities of CK3 (MM, 105 U/L), CK2 (MB, 6.8 U/L), CK1 (BB, 11 U/L), adenylate kinase (EC 2.7.4.3) anodal to CK3, and a fluorescent albumin artifact were found. Pooled plasma from neonates i...