To explore whether or not the gossypol varied in cottonseed by-products affect rumen degradability and fermentation efficiency, an vitro cumulative gas production experiment was applied with mixed microorganism to compare characteristics of whole (WCS, n = 3 samples), meal (CSM, hull (CSH, 2 samples). The five-replicate per sample incubation time continuously lasted for 0.5, 1.5, 3, 6, 12, 24, ...