Non-specific PCR amplification and DNA contamination usually accompany with PCR process, to overcome these problems, here we establish a sensor for thrombin by sequence-specific recognition of the PCR product with molecular beacon through triplex formation. Probe A and probe B were designed for the sensor, upon addition of thrombin, two probes hybridized to each other and the probe B was extend...

the antibiotic residues in milk are a well-known serious problem and pose several health hazards to consumers. we have described a simple, rapid, and inexpensive dllme-hplc/uv technique for the extraction of chloramphenicol and florfenicol residues in milk samples. under the optimum conditions, linearity of the method was observed over the range 0.02-0.85 µg/l with correlation coefficients > 0....

background: aflatoxin m1 (afm1) is a major carcinogenic compound that may be existed in milk and dairy products. this study was aimed to determine the presence of afm1 in industrial and traditional yogurt samples produced in iran. methods: a total of 80 yogurt samples (40 industrial, 40 traditional) was collected from supermarkets in yazd city, iran. afm1 content of the selected samples was det...

in recent years, the nanoscale zero-valent iron (nzvi) particles have been used successfully for the degradation of hydrocarboncompounds and remediation of other pollutants. nevertheless, as far as we know, there is no specific study on the improvement of thegeotechnical properties of contaminated soils with hydrocarbon compounds by nzvi. this study used nzvi particles to remove gasoil in a cla...

a fast and simple modified quechers (quick, easy, cheap, rugged and safe) extraction method based on spiked calibration curves and direct sample introduction was developed for determination of benzo[a]pyrene (bap) in bread by gas chromatography-mass spectrometry single quadrupole selected ion monitoring (gc/ms-sq-sim). sample preparation includes extraction of bap into acetone followed by clean...

The 1986 Chernobyl fallout had dramatic consequences for traditional reindeer husbandry in central and southern Norway, and also for many farmers in these areas. In end July 1986 the Government therefore passed a principal resolution stating that every producer should be economically compensated for all losses due to measures introduced by the authorities because of the Chernobyl deposition. By...

Trace element concentrations in blood plasma or serum have been assayed by numerous investigators using a variety of analytical techniques. For several elements, figures obtained in different centers are widely disparate. Impressive evidence has accumulated that a great deal of the inconsistencies should be ascribed to unsuspected contamination of the samples with exogenous material during thei...

Different definitions of risk too easily lead to differences in the type and level of risk accepted by varying stakeholders involved in the remediation process and redevelopment of contaminated sites as a whole. This can be rooted in the way risk information is interpreted and evaluated: technical risk definitions are largely evidential and based on engineering or technical contents, whereas so...

Alkylphenol polyethoxylate (APEO) derivatives, such as nonylphenol monoethoxylate (NP1EO), nonylphenol diethoxylate (NP2EO), nonylphenol (NP) and octylphenol (OP), have been detected in the surface water, sediment, food and groundwater of numerous countries. Because groundwater is the main source of water for pig herds, the aim of this study was to measure the concentrations of APEO derivatives...

Background: In 2010 contamination by dioxins and dioxin-like PCBs was detected in milk and meat in the valley Engidalur situated at the bottom of a fjord (Skutulsfjörður) in North West Iceland. The valley is narrow and surrounded by high mountains resulting in prevailing calm weather. The contamination was traced to a small municipal waste incinerator operating in the valley. Annual agricultura...