We have developed an electrophoretic method to determine the size of a protein—DNA complex using the same binding and electrophoresis conditions as in an electrophoretic mobility shift assay (EMSA). The method is an adaptation of that of Ferguson (1), originally developed to determine the size of native proteins in non-denaturing gel systems. In SDS polyacrylamide gel electrophoresis, associati...

rhizomania is the most important disease of sugar beet in iran and some other parts of the world, and plays an essential role in decreasing sugar yield in fields. the best approach to control this disease is to use resistant varieties. tagging the resistance genes in breeding programs, by molecular markers is necessary. in this study, some breeding populations and commercial varieties of sugar ...

of human tumors (1-10, 12-16). Since these extracts un doubtedly contain a spectrum of antigens, normal as well as tumor related, the isolation and purification of the TAA's would facilitate greatly the development of sensitive and specific immunological assays for the diagnosis and man agement of the cancer patient. Attempts to isolate the TAA's with the use of a variety of fractionation proce...

the domesticated silkworm, bombyx mori linn., a lepidopteran molecular model and an important economic insect that are emerging as an ideal molecular genetic resource for solving a broad range of biological problems. the silkworm, b. mori produces massive amount of silk proteins during the final stage of larval development. these proteins are stored in the middle silk gland and they are dischar...

background: failure in the treatment of burn patients infected with pseudomonas aeruginosa could happen as a result of the acquisition of antibiotic resistance, including carbapenems. objectives: the aim of the present study was to investigate the phenotypic and genotypic characteristics of the pseudomonas aeruginosa strains, isolated from burn patients. patients and methods: during a 12 month ...

morphological and electrophoretic analysis of the crystalline inclusions (parasporal crystals) of sporulated cultures of b. thuringiensis ssp. kumamotoensis dsm 6070 (bt 6070) was conducted via phase contrast and scanning electron microscopy and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. the activity of the spore-crystal suspensions against house fly (musca domestica) larvae wa...

stress is a threatening factor that all living organisms encounter throughout life. depending on the type of stress, there are several mechanisms for keeping body homeostasis to minimize stress effects. brain is an organ which shows high sensitivity to stress conditions. although many studies have shown induced-stress effects on rat embryos, little is known about the mechanisms involved in copi...

background and objective: acinetobacter baumannii is an aerobic non-motile gram-negative bacterial pathogen that is resistant to most antibiotics. carbapenems are the most common antibiotics for the treatment of infections caused by this pathogen. mechanisms of antibiotic-resistance in a. baumannii are mainly mediated by efflux pumps-lactamases. the aim of this study was to determine antibiotic...

a modified pulsed-field gel electrophoresis (pfge) protocol was developed and applied to clinical isolates of mycobacterium tuberculosis complex to reduce the cost of using lyticase. this protocol reduces the expense of pfge typing of mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.