Spectral imaging is a natural extension of the capabilities of confocal microscopes. The first confocal spectral imaging (CSI) instruments were able to acquire spectral data that allowed the emissions of overlapping fluorescent probes to be assigned to data channels representing a spectrum rather than a range of emission wavelengths. This marked a significant improvement over what could be done...

PURPOSE To evaluate maximum likelihood (ML) blind deconvolution as a technique for improving the repeatability of topographic height measurements obtained from scanning laser tomography (Heidelberg Retinal Tomograph [HRT]; Heidelberg Engineering, Heidelberg, Germany). METHODS ML blind deconvolution is an image-processing technique that estimates the original scene from a degraded image. This ...

introduction: leptin, secreted from adipose tissue may have some correlation with coronary artery disease according to some reports in literature. the mean concentration of plasma leptin in patients with normal and abnormal myocardial perfusion imaging (mpi) and relation of leptin levels, independently to abnormality in mpi after correction for body mass index (bmi) was evaluated. methods: as a...

the presence of anatomic variations of paranasal sinuses must be noted in order to attain a full understanding and accurate diagnosis of chronic sinusitis. the frequency of anatomic variations in chronic sinusitis is different in various studies. the purpose of this study is to assess the frequency of anatomic variations in chronic sinusitis patients admitted to otolaryngology ward of shahid sa...

introduction in ct imaging, metallic implants inside the tissues cause metal artifact that reduce the quality of image for diagnosis. in order to reduce the effect of this artifact, a new method with more appropriate results has been presented in this research work. materials and methods the presented method comprised of following steps: a) image enhancement and metal areas extraction, b) sinog...

Fast optical imaging of three-dimensional (3D) specimens is pivotal for the understanding of fundamental processes in life sciences. In most cases, 3D imaging is performed by acquiring a stack of 2D sections each at a different focal position. Notably, contrast and 3Dreconstruction accuracy are maximized with decreasing the optical thickness of 2D sections. This has spurred the development of s...

MOTIVATION Modern anatomical and developmental studies often require high-resolution imaging of large specimens in three dimensions (3D). Confocal microscopy produces high-resolution 3D images, but is limited by a relatively small field of view compared with the size of large biological specimens. Therefore, motorized stages that move the sample are used to create a tiled scan of the whole spec...

Electronic properties in different graphene materials are influenced by the presence of defects and their relative position with respect to the edges. Their localization is crucial for the reliable development of graphene-based electronic devices. Graphene samples produced by standard CVD on copper and by the scotch-tape method on gold were investigated using tip-enhanced Raman spectroscopy (TE...

Ectopic expression of the sarcoplasmic reticulum (SR) Ca(2+) ATPase (SERCA) 1a pump in the mouse heart results in a 2.5-fold increase in total SERCA pump level. SERCA1a hearts show increased rates of contraction/relaxation and enhanced Ca(2+) transients; however, the cellular mechanisms underlying altered Ca(2+) handling in SERCA1a transgenic (TG) hearts are unknown. In this study, using confoc...

3D Confocal Scanning Laser Microscopy to Quantify Contact Angles in Natural Oil-Water Mixtures — The contact angle of a liquid drop on a solid surface is one of the most simple, useful and sensitive parameters in the hydraulic sciences to quantify and to qualify the wettability and the surface energy of different materials. In this paper, a confocal scanning laser microscope (CSLM) has been use...